Oxymetholone modulates cell-mediated immunity in male B6C3F1 mice

被引:7
作者
Karrow, NA [1 ]
McCay, JA [1 ]
Brown, R [1 ]
Musgrove, D [1 ]
Munson, AE [1 ]
White, KL [1 ]
机构
[1] Virginia Commonwealth Univ, Dept Pharmacol & Toxicol, Richmond, VA 23298 USA
关键词
oxymetholone; immunomodulation; cell-mediated immunity; mice;
D O I
10.1081/DCT-100101974
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Oxymetholone is a synthetic androgen, structurally related to testosterone. It is currently used to treat anemias, but has also been abused as a performance enhancing anabolic steroid by the sport community. Concern about its suspected immunomodulatory properties provided the incentive for a detailed investigation into its effects on the mammalian immune system. In this study, male B6C3F1 mice were treated for 14 d with oxymetholone (0, 50, 150, and 300 mg/kg) by gastric intubation, then evaluated for immunotoxicity using a panel of immunotoxicity assays. Except for an increasing trend in kidney and liver weights, and a dose-dependent increase in serum blood urea nitrogen levels, no other signs of systemic toxicity were observed. Bone marrow DNA synthesis was reduced, though this did not translate into alterations in myeloid or monocyte colony forming units. Spleen B and T cell numbers, antibody response to sheep red blood cells, proliferative response to both mitogen and immunoglobulin receptor immunogens, and NK cell activity were all unaltered in mice treated with oxymetholone. Peritoneal macrophage activity was also unaffected by oxymetholone treatment. A 38% decrease in the spleen cell mixed leukocyte response, and a 15% decrease in cytotoxic T cell activity, measured in the highest oxymetholone treatment group, indicate that cell-mediated immunity was impaired following exposure. This immunomodulation did not however, translate into a change in host resistance to Listeria monocytogenes.
引用
收藏
页码:621 / 644
页数:24
相关论文
共 50 条
  • [41] Comparison of tumorigenesis between accelerated heavy ion and X-ray in B6C3F1 mice
    Watanabe, H
    Ogiu, T
    Nishimura, M
    Masaoka, Y
    Kurosumi, M
    Takahashi, T
    Oguri, T
    Shoji, S
    Katoh, O
    [J]. JOURNAL OF RADIATION RESEARCH, 1998, 39 (02) : 93 - 100
  • [42] Evaluation of the immunomodulatory effects of the disinfection by-product, sodium chlorite, in female B6C3F1 mice: A drinking water study
    Karrow, NA
    Guo, TL
    McCay, JA
    Johnson, GW
    Brown, RD
    Musgrove, DL
    Germolec, DR
    Luebke, RW
    White, KL
    [J]. DRUG AND CHEMICAL TOXICOLOGY, 2001, 24 (03) : 239 - 258
  • [43] A PSEUDOLIPOMA ON THE DIAPHRAGMATIC SURFACE OF THE LIVER IN A FEMALE B6C3F1 MOUSE
    YOSHITOMI, K
    BOORMAN, GA
    [J]. VETERINARY PATHOLOGY, 1993, 30 (02) : 209 - 211
  • [44] Effects of metal working fluids on B6C3F1 mouse skin
    Al-Humadi, NH
    Battelli, L
    Willard, P
    Schwegler-Berry, D
    Castranova, V
    Kommineni, C
    [J]. TOXICOLOGY AND INDUSTRIAL HEALTH, 2000, 16 (06) : 203 - 210
  • [45] Forestomach tumor induction by 2,4-hexadienal in F344N rats and B6C3F1 mice
    Po C. Chan
    Joel Mahler
    Shyamal Peddada
    Liat Lomnitski
    Abraham Nyska
    [J]. Archives of Toxicology, 2003, 77 : 511 - 520
  • [46] TOXICITY OF MONOCHLOROACETIC ACID ADMINISTERED BY GAVAGE TO F344 RATS AND B6C3F1 MICE FOR UP TO 13 WEEKS
    BRYANT, BJ
    JOKINEN, MP
    EUSTIS, SL
    THOMPSON, MB
    ABDO, KM
    [J]. TOXICOLOGY, 1992, 72 (01) : 77 - 87
  • [47] Cigarette smoke exposure produces more evidence of emphysema in B6C3F1 mice than in F344 rats
    March, TH
    Barr, EB
    Finch, GL
    Hahn, FF
    Hobbs, CH
    Ménache, MG
    Nikula, KJ
    [J]. TOXICOLOGICAL SCIENCES, 1999, 51 (02) : 289 - 299
  • [48] Two-year toxicity and carcinogenicity study of methyleugenol in F344/N rats and B6C3F1 mice
    Johnson, JD
    Ryan, MJ
    Toft, JD
    Graves, SW
    Hejtmancik, MR
    Cunningham, ML
    Herbert, R
    Abdo, KM
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (08) : 3620 - 3632
  • [49] Forestomach tumor induction by 2,4-hexadienal in F344N rats and B6C3F1 mice
    Chan, PC
    Mahler, J
    Peddada, S
    Lomnitski, L
    Nyska, A
    [J]. ARCHIVES OF TOXICOLOGY, 2003, 77 (09) : 511 - 520
  • [50] EFFECTS OF A 13-WEEK CHLOROPENTAFLUOROBENZENE INHALATION EXPOSURE OF FISCHER 344 RATS AND B6C3F1 MICE
    KINKEAD, ER
    BUNGER, SK
    KIMMEL, EC
    FLEMMING, CD
    WALL, HG
    GRABAU, JH
    [J]. TOXICOLOGY AND INDUSTRIAL HEALTH, 1991, 7 (04) : 309 - 318