The UVB-induced synthesis of vitamin D3 and 1α,25-dihydroxyvitamin D3 (calcitriol) in organotypic cultures of keratinocytes:: Effectiveness of the narrowband Philips TL-01 lamp (311 nm)

被引:23
作者
Lehmann, Bodo [1 ]
Knuschke, Peter [1 ]
Meurer, Michael [1 ]
机构
[1] Tech Univ Dresden, Carl Gustav Carus Med Sch, Dept Dermatol, D-01307 Dresden, Germany
关键词
UVB; 7-dehydrocholesterol; vitamin D-3; calcitriol; keratinocyte; metabolism;
D O I
10.1016/j.jsbmb.2006.12.033
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Both calcitriol and UVB radiation exert potent antipsoriatic effects. We hypothesize that the therapeutical effect of UVB radiation may be attributed at least in part to UVB-triggered cutaneous synthesis of calcitriol. The optimum wavelength for initiation of the vitamin D-3 pathway was found to be in the range of 300 +/- 5 nm in vitro and in vivo. The narrowband Philips TL-01 lamp which is commonly used as UVB source for phototherapy of psoriasis has maximum spectral irradiance at around 311 nm which is presumed to be, however, of lesser importance in photochemical activation of the vitamin D3 pathway. The aim of this study was to compare the vitamin D-3 and calcitriol-inducing potential of UVB from the TL-01 lamp with that of monochromatic UVB at 300 +/- 2.5 nm and 310 +/- 2.5 nm in organotypic cultures of keratinocytes supplemented with 25 mu M 7-DHC. We found that maximum calcitriol-generating capacity of the TL-01 lamp at 500 mJ/cm(2) and 16 h after irradiation still amounts up to 44% of that found after monochromatic irradiation at 300 +/- 2.5 nm and 30 mJ/cm(2). Thus, the antipsoriatic effect of UVB emitted from the TL-01 lamp may, at least in part, based on the antiproliferative and prodifferentiative action of newly synthesized calcitriol on epidermal keratinocytes. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:682 / 685
页数:4
相关论文
共 16 条
  • [1] BARTH J, 1991, AKTUEL DERMATOL, V17, P319
  • [2] 3II-NM UVB PHOTOTHERAPY - AN EFFECTIVE TREATMENT FOR PSORIASIS
    GREEN, C
    FERGUSON, J
    LAKSHMIPATHI, T
    JOHNSON, BE
    [J]. BRITISH JOURNAL OF DERMATOLOGY, 1988, 119 (06) : 691 - 696
  • [3] HOLICK MF, 1989, J INVEST DERMATOL, V92, P446
  • [4] REGULATION OF CUTANEOUS PREVITAMIN-D3 PHOTOSYNTHESIS IN MAN - SKIN PIGMENT IS NOT AN ESSENTIAL REGULATOR
    HOLICK, MF
    MACLAUGHLIN, JA
    DOPPELT, SH
    [J]. SCIENCE, 1981, 211 (4482) : 590 - 593
  • [5] KNUSCHKE P, 1996, OHOTODERMATOL PHOTOI, V12, P251
  • [6] TOPICAL CALCITRIOL IN THE TREATMENT OF CHRONIC PLAQUE PSORIASIS - A DOUBLE-BLIND-STUDY
    LANGNER, A
    VERJANS, H
    STAPOR, V
    MOL, M
    FRACZYKOWSKA, M
    [J]. BRITISH JOURNAL OF DERMATOLOGY, 1993, 128 (05) : 566 - 571
  • [7] Demonstration of UVB-induced synthesis of 1α,25-dihydroxyvitamin D3 (calcitriol) in human skin by microdialysis
    Lehmann, B
    Sauter, W
    Knuschke, P
    Dressler, S
    Meurer, M
    [J]. ARCHIVES OF DERMATOLOGICAL RESEARCH, 2003, 295 (01) : 24 - 28
  • [8] UVB-induced conversion of 7-dehydrocholesterol to 1α,25-dihydroxyvitamin D3 in an in vitro human skin equivalent model
    Lehmann, B
    Genehr, T
    Knuschke, P
    Pietzsch, J
    Meurer, M
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2001, 117 (05) : 1179 - 1185
  • [9] Lehmann B, 2000, PHOTOCHEM PHOTOBIOL, V72, P803, DOI 10.1562/0031-8655(2000)072&lt
  • [10] 0803:UICODT&gt