Metabolic Fingerprint of Brazilian Maize Landraces Silk (Stigma/Styles) Using NMR Spectroscopy and Chemometric Methods

被引：24

作者：

Kuhnen, Shirley ^{[2
]}

Ogliari, Juliana Bernardi ^{[3
]}

Dias, Paulo Fernando ^{[4
]}

Santos, Maiara da Silva ^{[5
]}

Ferreira, Antonio Gilberto ^{[5
]}

Bonham, Connie C. ^{[6
]}

Wood, Karl Vernon ^{[6
]}

Maraschin, Marcelo ^{[1
]}

机构：

[1] Univ Fed Santa Catarina, Dept Plant Sci, Plant Morphogenesis & Biochem Lab, BR-88049900 Florianopolis, SC, Brazil

[2] Univ Fed Mato Grosso, Dept Chem, Biochem Lab, BR-78060090 Cuiaba, MT, Brazil

[3] Univ Fed Santa Catarina, Dept Plant Sci, Lab Res Agrobiodivers, BR-88049900 Florianopolis, SC, Brazil

[4] Univ Fed Santa Catarina, Dept Cell Biol Embryol & Genet, Florianopolis, SC, Brazil

[5] Univ Fed Sao Carlos, Dept Chem, BR-13560 Sao Carlos, SP, Brazil

[6] Purdue Univ, Dept Biochem, Campus Wide Mass Spectrometry Ctr, W Lafayette, IN 47907 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2010年 / 58卷 / 04期

关键词：

Maize silks; landraces; anthocyanins; polyphenols; H-1 NMR spectroscopy; principal component analysis; metabolomics; ZEA-MAYS; MULTIVARIATE-ANALYSIS; ANTIOXIDANT ACTIVITIES; H-1-NMR SPECTROSCOPY; EXTRACTS; ANTHOCYANINS;

D O I：

10.1021/jf9037776

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Aqueous extract from maize silks is used by traditional medicine for the treatment of several ailments, mainly related to the urinary system. This work focuses on the application of NMR spectroscopy and chemometric analysis for the determination of metabolic fingerprint and pattern recognition of silk extracts from seven maize landraces cultivated in southern Brazil. Principal component analysis (PCA) of the H-1 NMR data set showed clear discrimination among the maize varieties by PC1 and PC2, pointing out three distinct metabolic profiles, Target compounds analysis showed significant differences (p<0.05) in the contents of protocatechuic acid, gallic acid, t-cinnamic acid, and anthocyanins, corroborating the discrimination of the genotypes in this study as revealed by PCA analysis. Thus the combination of H-1 NMR and PCA is a useful tool for the discrimination of maize silks in respect to their chemical composition, including rapid authentication of the raw material of current pharmacological interest.

引用

页码：2194 / 2200

页数：7

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