Generation of medaka gene knockout models by target-selected mutagenesis

被引：111
作者：
Taniguchi, Yoshihito
Takeda, Shunichi
Furutani-Seiki, Makoto
Kamei, Yasuhiro
Todo, Takeshi
Sasado, Takao
Deguchi, Tomonori
Kondoh, Hisato
Mudde, Josine
Yamazoe, Mitsuyoshi
Hidaka, Masayuki
Mitani, Hiroshi
Toyoda, Atsushi
Sakaki, Yoshiyuki
Plasterk, Ronald H. A.
Cuppen, Edwin
机构：
[1] Netherlands Inst Dev Biol, Hubrecht Lab, NL-3584 CT Utrecht, Netherlands
[2] Kyoto Univ, CREST, Japan Sci & Technol Lab, Dept Radiat Genet,Sakyo Ku, Kyoto 6068501, Japan
[3] Japan Sci & Technol Corp, ERATO, Kondoh Differentiat Signaling Project, Sakyo Ku, Kyoto 6068305, Japan
[4] Kyoto Univ, Ctr Radiat Biol, Dept Mutagenesis, Kyoto 6068501, Japan
[5] Univ Tokyo, Dept Integrated Biosci, Kashiwa, Chiba 2778562, Japan
[6] RIKEN, Yokohama Inst, Inst Phys & Chem Res, Genom Sci Ctr, Yokohama, Kanagawa 2300045, Japan
来源：
GENOME BIOLOGY | 2006年 / 7卷 / 12期
关键词：
D O I：
10.1186/gb-2006-7-12-r116
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have established a reverse genetics approach for the routine generation of medaka ( Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea ( ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes.
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页数：14
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