A new efficient expression system for Bacillus and its application to production of recombinant phytase

被引：27
|
作者：
Kerovuo, J [1 ]
von Weymarn, N
Povelainen, M
Auer, S
Miasnikov, A
机构：
[1] Danisco Cultor Innovat, Kantvik Res Ctr, Kantvik, Finland
[2] Helsinki Univ Technol, Lab Bioproc Engn, FIN-02150 Espoo, Finland
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 16期
关键词：
Bacillus subtilis; fed-batch cultivation; phosphate-starvation; phytase; plasmid;
D O I：
10.1023/A:1005694731039
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A new expression system was developed for Bacillus subtilis. This system uses a shuttle vector (B. subtilis - Eschericia coli) carrying a phosphate starvation-inducible promoter (pst) and on a fed-batch cultivation strategy. The pst-promoter proved to be very strong and retain its tight regulation also when present on a multi-copy plasmid. The expression system developed showed promising results when applied to the production of recombinant Bacillus phytase - phytase activity at the end of cultivation reached 28.7 U ml(-1).
引用
收藏
页码：1311 / 1317
页数：7
相关论文
共 50 条
  • [21] Strategy for efficient production of recombinant Staphylococcus epidermidis lipase in Bacillus subtilis
    Chen, Po Ting
    Chen, Yi-Chung
    Lin, Yong-Yang
    Su, Heng-Hui
    BIOCHEMICAL ENGINEERING JOURNAL, 2015, 103 : 152 - 157
  • [22] Efficient Autoinducible Expression of Recombinant Proteins via the DegSU Quorum Sensing System in a Robust Bacillus subtilis
    Zong, Hong
    Zhang, Liya
    Cheng, Yiwen
    Sheng, Zhiying
    Bin, Zhuge
    Lu, Xinyao
    ACS SYNTHETIC BIOLOGY, 2025, 14 (01): : 273 - 284
  • [23] Heterologous expression of cyclodextrin glycosyltransferase from Bacillus stearothermophilus in Bacillus subtilis and its application in glycosyl rutin production
    Song, Wen
    Zhang, Mengjie
    Li, Xiaojun
    Zhang, Yinjun
    Zheng, Jianyong
    3 BIOTECH, 2023, 13 (03)
  • [24] Efficient production of recombinant IgG by the GLUT5 co-expression system
    Yuichi Inoue
    Aiko Inoue
    Hiroharu Kawahara
    BMC Proceedings, 5 (Suppl 8)
  • [25] Heterologous expression of cyclodextrin glycosyltransferase from Bacillus stearothermophilus in Bacillus subtilis and its application in glycosyl rutin production
    Wen Song
    Mengjie Zhang
    Xiaojun Li
    Yinjun Zhang
    Jianyong Zheng
    3 Biotech, 2023, 13
  • [26] Production of recombinant human tissue transglutaminase using the baculovirus expression system, and its application for serological diagnosis of coeliac disease
    Osman, AA
    Richter, T
    Stern, M
    Conrad, K
    Henker, J
    Brandsch, C
    Zimmer, KP
    Mothes, T
    EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY, 2002, 14 (11) : 1217 - 1223
  • [27] New Efficient Recombinant Expression System To Engineer Candida antarctica Lipase B
    Emond, Stephane
    Montanier, Cedric
    Nicaud, Jean-Marc
    Marty, Alain
    Monsan, Pierre
    Andre, Isabelle
    Remaud-Simeon, Magali
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (08) : 2684 - 2687
  • [28] Expression and characterization of extreme alkaline, oxidation-resistant keratinase from Bacillus licheniformis in recombinant Bacillus subtilis WB600 expression system and its application in wool fiber processing
    Liu, Baihong
    Zhang, Juan
    Li, Ben
    Liao, Xiangru
    Du, Guocheng
    Chen, Jian
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2013, 29 (05): : 825 - 832
  • [29] Expression and characterization of extreme alkaline, oxidation-resistant keratinase from Bacillus licheniformis in recombinant Bacillus subtilis WB600 expression system and its application in wool fiber processing
    Baihong liu
    Juan Zhang
    Ben Li
    Xiangru Liao
    Guocheng Du
    Jian Chen
    World Journal of Microbiology and Biotechnology, 2013, 29 : 825 - 832
  • [30] A Bacillus megaterium System for the Production of Recombinant Proteins and Protein Complexes
    Biedendieck, Rebekka
    ADVANCED TECHNOLOGIES FOR PROTEIN COMPLEX PRODUCTION AND CHARACTERIZATION, 2016, 896 : 97 - 113
12345