A new efficient expression system for Bacillus and its application to production of recombinant phytase

被引：27
|
作者：
Kerovuo, J [1 ]
von Weymarn, N
Povelainen, M
Auer, S
Miasnikov, A
机构：
[1] Danisco Cultor Innovat, Kantvik Res Ctr, Kantvik, Finland
[2] Helsinki Univ Technol, Lab Bioproc Engn, FIN-02150 Espoo, Finland
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 16期
关键词：
Bacillus subtilis; fed-batch cultivation; phosphate-starvation; phytase; plasmid;
D O I：
10.1023/A:1005694731039
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A new expression system was developed for Bacillus subtilis. This system uses a shuttle vector (B. subtilis - Eschericia coli) carrying a phosphate starvation-inducible promoter (pst) and on a fed-batch cultivation strategy. The pst-promoter proved to be very strong and retain its tight regulation also when present on a multi-copy plasmid. The expression system developed showed promising results when applied to the production of recombinant Bacillus phytase - phytase activity at the end of cultivation reached 28.7 U ml(-1).
引用
收藏
页码：1311 / 1317
页数：7
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