Discovery of 3-[2-(Imidazo[1,2-b]pyridazin-3-yl)ethynyl]-4-methyl-N-{4-[(4-methylpiperazin-1-yl)methyl]-3-(trifluoromethyl)phenyl}benzamide (AP24534), a Potent, Orally Active Pan-Inhibitor of Breakpoint Cluster Region-Abelson (BCR-ABL) Kinase Including the T315I Gatekeeper Mutant

被引：307

作者：

Huang, Wei-Sheng ^{[1
]}

Metcalf, Chester A. ^{[1
]}

Sundaramoorthi, Raji ^{[1
]}

Wang, Yihan ^{[1
]}

Zou, Dong ^{[1
]}

Thomas, R. Mathew ^{[1
]}

Zhu, Xiaotian ^{[1
]}

Cai, Lisi ^{[1
]}

Wen, David ^{[1
]}

Liu, Shuangying ^{[1
]}

Romero, Jan ^{[1
]}

Qi, Jiwei ^{[1
]}

Chen, Ingrid ^{[1
]}

Banda, Geetha ^{[1
]}

Lentini, Scott P. ^{[1
]}

Das, Sasmita ^{[1
]}

Xu, Qihong ^{[1
]}

Keats, Jeff ^{[1
]}

Wang, Frank ^{[1
]}

Wardwell, Scott ^{[1
]}

Ning, Yaoyu ^{[1
]}

Snodgrass, Joseph T. ^{[1
]}

Broudy, Marc I. ^{[1
]}

Russian, Karin ^{[1
]}

Zhou, Tianjun ^{[1
]}

Commodore, Lois ^{[1
]}

Narasimhan, Narayana I. ^{[1
]}

Mohemmad, Qurish K. ^{[1
]}

Iuliucci, John ^{[1
]}

Rivera, Victor M. ^{[1
]}

Dalgarno, David C. ^{[1
]}

Sawyer, Tomi K. ^{[1
]}

Clackson, Tim ^{[1
]}

Shakespeare, William C. ^{[1
]}

机构：

[1] ARIAD Pharmaceut Inc, Cambridge, MA 02139 USA

来源：

JOURNAL OF MEDICINAL CHEMISTRY | 2010年 / 53卷 / 12期

关键词：

CHRONIC MYELOID-LEUKEMIA; CENTRAL-NERVOUS-SYSTEM; IN-VIVO ACTIVITY; TYROSINE KINASE; SELECTIVE INHIBITOR; CRYSTAL-STRUCTURE; AURORA KINASES; IMATINIB; RESISTANCE; MUTATIONS;

D O I：

10.1021/jm100395q

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

In the treatment of chronic myeloid leukemia (CML) with BCR-ABL kinase inhibitors, the T315I gatekeeper mutant has emerged as resistant to all currently approved agents. This report describes the structure-guided design of a novel series of potent pan-inhibitors of BCR-ABL, including the T315I mutation. A key structural feature is the carbon-carbon triple bond linker which skirts the increased bulk of I1e315 side chain. Extensive SAR studies led to the discovery of development candidate 20g (AP24534), which inhibited the kinase activity of both native BCR-ABL and the T315I mutant with low nM IC(50)s, and potently inhibited proliferation of corresponding Ba/F3-derived cell lines. Daily oral administration of 20g significantly prolonged survival of mice injected intravenously with BCR-ABL(T315I) expressing Ba/F3 cells. These data, coupled with a favorable ADME profile, support the potential of 20g to be an effective treatment for CM L, including patients refractory to all currently approved therapies.

引用

页码：4701 / 4719

页数：19

共 56 条

[41] Imatinib and beyond-exploring the full potential of targeted therapy for CML [J].