Structural analysis of the catalytic and binding sites of Clostridium botulinum neurotoxin B

被引：365
作者：
Swaminathan, S [1 ]
Eswaramoorthy, S [1 ]
机构：
[1] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA
来源：
NATURE STRUCTURAL BIOLOGY | 2000年 / 7卷 / 08期
关键词：
D O I：
10.1038/78005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Clostridium botulinum neurotoxins are among the most potent toxins to humans. The crystal structures of intact C. botulinum neurotoxin type B (BoNT/B) and its complex with sialyllactose, determined at 1.8 and 2.6 Angstrom resolution, respectively, provide insight into its catalytic and binding sites. The position of the belt region in BoNT/B is different from that in BoNT/A; this observation presents interesting possibilities for designing specific inhibitors that could be used to block the activity of this neurotoxin. The structures of BoNT/B and its complex with sialyllactose provide a detailed description of the active site and a model for interactions between the toxin and its cell surface receptor. The latter may provide valuable information for recombinant vaccine development.
引用
收藏
页码：693 / 699
页数：7
相关论文
共 51 条
[1]   STRUCTURE OF EXOTOXIN-A OF PSEUDOMONAS-AERUGINOSA AT 3.0-ANGSTROM RESOLUTION [J].
ALLURED, VS ;
COLLIER, RJ ;
CARROLL, SF ;
MCKAY, DB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (05) :1320-1324
[2]   THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].
BAILEY, S .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763
[3]  
BLASI J, 1993, EMBO J, V12, P4821, DOI 10.1002/j.1460-2075.1993.tb06171.x
[4]   Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].
Brunger, AT ;
Adams, PD ;
Clore, GM ;
DeLano, WL ;
Gros, P ;
Grosse-Kunstleve, RW ;
Jiang, JS ;
Kuszewski, J ;
Nilges, M ;
Pannu, NS ;
Read, RJ ;
Rice, LM ;
Simonson, T ;
Warren, GL .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921
[5]   RIBBONS 2 0 [J].
CARSON, M .
JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1991, 24 :958-&
[6]   THE CRYSTAL-STRUCTURE OF DIPHTHERIA-TOXIN [J].
CHOE, S ;
BENNETT, MJ ;
FUJII, G ;
CURMI, PMG ;
KANTARDJIEFF, KA ;
COLLIER, RJ ;
EISENBERG, D .
NATURE, 1992, 357 (6375) :216-222
[7]   Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods [J].
delaFortelle, E ;
Bricogne, G .
MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 :472-494
[8]   PHASES-95: A program package for processing and analyzing diffraction data from macromolecules [J].
Furey, W ;
Swaminathan, S .
MACROMOLECULAR CRYSTALLOGRAPHY, PT B, 1997, 277 :590-+
[9]   Streptomyces griseus aminopeptidase: X-ray crystallographic structure at 1.75 angstrom resolution [J].
Greenblatt, HM ;
Almog, O ;
Maras, B ;
SpunginBialik, A ;
Barra, D ;
Blumberg, S ;
Shoham, G .
JOURNAL OF MOLECULAR BIOLOGY, 1997, 265 (05) :620-636
[10]  
HALPERN JL, 1993, J BIOL CHEM, V268, P11188
123456