A new laccase from dried fruiting bodies of the monkey head mushroom Hericium erinaceum

被引：61
作者：
Wang, H
Ng, TB [1 ]
机构：
[1] Chinese Univ Hong Kong, Fac Med, Dept Biochem, Shatin, Hong Kong, Peoples R China
[2] China Agr Univ, Dept Microbiol, Beijing, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 322卷 / 01期
关键词：
laccase; HIV; mushroom;
D O I：
10.1016/j.bbrc.2004.07.075
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A laccase with a novel N-terminal sequence, a molecular mass of 63 kDa, and inhibitory activity toward HIV-1 reverse transcriptase (IC50 = 9.5 muM) was isolated from dried fruiting bodies of the monkey head mushroom Hericium erinaceum. A chromatographic procedure involving ion exchange chromatography on DEAE-cellulose, CM-cellulose, and Q-Sepharose and fast protein liquid chromatography-gel filtration on Superdex 75 was employed. The laccase was adsorbed on DEAE-cellulose and Q-Sepharose but unadsorbed on CM-cellulose. High activity of the enzyme was observed at pH 3-5 and at 50-80 degreesC. Its activity was completely abolished at pH 8 and 9 and after boiling for 10 min. A temperature of 50 degreesC and a pH of 5.0 were optimal for its activity. (C) 2004 Elsevier Inc. All rights reserved.
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收藏
页码：17 / 21
页数：5
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