Karyological investigation of Calotropis gigantea (L.) W. T. Aiton and Centella asiatica (L.) Urban: Two important medicinal plants of Bangladesh

被引:1
|
作者
Dash, Chandan Kumar [1 ]
Rahman, Md. Shahidur [2 ]
Afroz, Mahin [3 ]
Sultana, Syeda Sharmeen [1 ]
机构
[1] Univ Dhaka, Dept Bot, Cytogenet Lab, Dhaka 1000, Bangladesh
[2] Bangladesh Open Univ, Open Sch, Gazipur 1705, Bangladesh
[3] Barishal Univ, Dept Bot, Barishal 8200, Bangladesh
来源
JOURNAL OF APPLIED RESEARCH ON MEDICINAL AND AROMATIC PLANTS | 2019年 / 14卷
关键词
Chromosome; CMA; DAPI; Karyotype; Calotropis gigantea; Centella asiatica; CHROMOSOME-NUMBERS; KARYOTYPE ANALYSIS;
D O I
10.1016/j.jarmap.2019.100221
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Calotropis gigantea (L.) W. T. Aiton and Centella asiatica (L.) Urban have been medicinally used throughout Bangladesh. In this study, karyotypic features of these two species were studied with orcein staining and banding with two base specific fluorochromes such as chromomycine A(3) (CMA) and 4', 6 diamidino-2-phenylindole (DAPI) for authentic cytogenetical characterization. 2n = 22 and 2n = 18 somatic chromosomes were found in C. gigantea and C. asiatica, respectively. The total chromosome length of C. gigantea and C. asiatica were 45.35 +/- 2.64 mu m and 63.63 +/- 3.19 mu m, respectively. The total form percent (TF%), karyotype asymmetry index (AsK%) and karyotype symmetry index (Syi index) were 47.61, 52.39 and 90.87 in C. gigantea and 39.58, 60.42 and 65.51 in C. asiatica, respectively. Both species were found to possess 1A karyotype. In C. gigantea, 2 CMA- and 4 DAPI-bands were observed whereas C. asiatica had 16 CMA- and 6 DAPI-bands. Therefore, the compiled data generated from chromosome analysis on orcein, CMA- and DAPI-banding will be useful for cytogenetical characterization of these two species.
引用
收藏
页数:5
相关论文
共 50 条
  • [1] Pharmacognostic Specifications of Calotropis procera (Aiton) W. T. Aiton Leaf and Tribulus terrestris L. Fruit: Important Medicinal Plants Used in Veterinary Products
    Monika
    Akanksha
    Lal, Snigdha
    JOURNAL OF PHARMACEUTICAL RESEARCH INTERNATIONAL, 2021, 33 (47A) : 93 - 103
  • [2] Exotic Medicinal Plants (7) Centella asiatica (L.) Urban (Apiaceae)
    Jeannin, Jean-Michel
    SCHWEIZERISCHE ZEITSCHRIFT FUER GANZHEITSMEDIZIN, 2011, 23 (01): : 25 - 25
  • [3] Mutational breeding of Centella asiatica (L.) urban for medicinal purposes
    Kaensaksiri, T.
    Soontornchainaksaeng, P.
    Soonthornchareonnon, N.
    Prathanturarug, S.
    PLANTA MEDICA, 2010, 76 (12) : 1238 - 1238
  • [4] Pentacyclic Triterpenoids from the Medicinal Herb, Centella asiatica (L.) Urban
    James, Jacinda T.
    Dubery, Ian A.
    MOLECULES, 2009, 14 (10): : 3922 - 3941
  • [5] Callus culture and micropropagation of Hydrocotyle asiatica [Centella asiatica (L.) Urban], a medicinal plant
    Josekutty, PC
    PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY, 1998, 63 (1-2) : 275 - 278
  • [6] Response of Matthiola incana (L.) W. T. Aiton to Cultivation in the Mediterranean Climate
    Scuderi, D.
    Toscano, S.
    Romano, D.
    VI INTERNATIONAL SYMPOSIUM ON BRASSICAS AND XVIII CRUCIFER GENETICS WORKSHOP, 2013, 1005 : 511 - 517
  • [7] Somatic embryogenesis in Centella asiatica L. an important medicinal and neutraceutical plant of India
    Ch. Paramageetham
    G. Prasad Babu
    J.V.S. Rao
    Plant Cell, Tissue and Organ Culture, 2004, 79 : 19 - 24
  • [8] Somatic embryogenesis in Centella asiatica L. an important medicinal and neutraceutical plant of India
    Paramageetham, C
    Babu, GP
    Rao, JVS
    PLANT CELL TISSUE AND ORGAN CULTURE, 2004, 79 (01) : 19 - 24
  • [9] Molecular, chemical and biological stability of plants derived from artificial seeds of Centella asiatica (L.) Urban-An industrially important medicinal herb
    Prasad, Archana
    Singh, Manju
    Yadav, Narayan Prasad
    Mathur, Ajay Kumar
    Mathur, Archana
    INDUSTRIAL CROPS AND PRODUCTS, 2014, 60 : 205 - 211
  • [10] Growth stage-specific Accumulation of Cardiac Glycosides in Two Variants of Calotropis gigantea (L.) WT Aiton
    Singh, Pankaj
    Singh, Yatendra
    Kanojiya, Sanjeev
    Tripathi, Vineeta
    Mishra, Dipak Kumar
    PHARMACOGNOSY MAGAZINE, 2022, 18 (78) : 353 - 359