Establishment of an analyzing method for a Japanese encephalitis virus neutralization test in Vero cells

被引：19

作者：

Abe, M ^{[1
]}

Kuzuhara, S ^{[1
]}

Kino, Y ^{[1
]}

机构：

[1] Chemo Sero Therapeut Res Inst, Kikuchi Res Ctr, Kumamoto 8691298, Japan

来源：

VACCINE | 2003年 / 21卷 / 17-18期

关键词：

Japanese encephalitis virus; neutralization test; Vero cells;

D O I：

10.1016/S0264-410X(02)00772-7

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We established a 50% plaque reduction analyzing method of neutralizing antibody for human serum to Japanese encephalitis virus (JEV) in Vero cells, called the '3 points least-squares regression method' (3LSRM). Our method shows a high correlation with the chick embryo cell method (the current standard method for human serum), using the chart method established by the National Institute of Infectious Diseases of Japan, which is an equation made with retrospective data obtained with the 50% plaque reduction method as a standard measurement method for neutralizing antibody titers to JEV. Our new method is much simpler and more reliable than the current method in that it uses an established cell line, Vero cells, and its results are computed by the 3LSRM. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1989 / 1994

页数：6

共 25 条

[1] PROTECTION AGAINST JAPANESE ENCEPHALITIS BY INACTIVATED VACCINES [J].

HOKE, CH ;

NISALAK, A ;

SANGAWHIPA, N ;

JATANASEN, S ;

LAORAKAPONGSE, T ;

INNIS, BL ;

KOTCHASENEE, SO ;

GINGRICH, JB ;

LATENDRESSE, J ;

FUKAI, K ;

BURKE, DS .

NEW ENGLAND JOURNAL OF MEDICINE, 1988, 319 (10) :608-614

[2]

HORAUD F, 1992, DEV BIOLOGICALS, V76, P43

[3]

KIMURAKURODA J, 1988, J IMMUNOL, V141, P3606

[4] ANTIGENIC COMPARISON OF ENVELOPE PROTEIN-E BETWEEN JAPANESE ENCEPHALITIS-VIRUS AND SOME OTHER FLAVIVIRUSES USING MONOCLONAL-ANTIBODIES [J].

KIMURAKURODA, J ;

YASUI, K .

JOURNAL OF GENERAL VIROLOGY, 1986, 67 :2663-2672

[5]

KITANO T, 1996, VACCINE HDB, P103

[6] Immunogenicity and protective efficacy of the current inactivated Japanese encephalitis vaccine against different Japanese encephalitis virus strains [J].

Kurane, I ;

Takasaki, T .

VACCINE, 2000, 18 :33-35

[7]

Mackenzie J.S., 1999, CLIN VIROL, V27, P1

[8]

Mackenzie JS, 1997, FACTORS IN THE EMERGENCE OF ARBOVIRUS DISEASES, P191

[9] Points to consider in the development of a surrogate for efficacy of novel Japanese encephalitis virus vaccines [J].

Markoff, L .

VACCINE, 2000, 18 :26-32

[10]

MONTAGNON B, 1983, DEV BIOLOGICALS, V55, P37

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