Application of a Metal-Mediated Base Pair to the Detection of Medicinally Relevant Single Nucleotide Polymorphisms

被引：33

作者：

Jash, Biswarup ^{[1
,2
]}

Mueller, Jens ^{[1
,2
]}

机构：

[1] Westfalische Wilhelms Univ Munster, Inst Anorgan & Analyt Chem, Corrensstr 28-30, D-48149 Munster, Germany

[2] Westfalische Wilhelms Univ Munster, NRW Grad Sch Chem, Corrensstr 28-30, D-48149 Munster, Germany

来源：

EUROPEAN JOURNAL OF INORGANIC CHEMISTRY | 2017年 / 33期

关键词：

DNA; Silver; Metal-mediated base pairs; Single nucleotide polymorphism; Nucleobases; CHARGE-TRANSFER; ARTIFICIAL DNA; 3-WAY JUNCTIONS; NUCLEIC-ACIDS; STRANDED DNA; NUCLEOBASES; DUPLEX; GENE; HYBRIDIZATION; ASSOCIATION;

D O I：

10.1002/ejic.201700665

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

A highly stabilizing metal-mediated base pair formed from the canonical nucleobase cytosine (C), the transition metal ion Ag-I, and the artificial nucleobase 1H-imidazo[4,5-f]-[1,10] phenanthroline (P) was used in the generation of molecular beacons for the detection of medicinally relevant single nucleotide polymorphisms (SNPs) based on either a C -> T or a T -> C transition (T: thymine). The beacons exploit the fact that a P-C pair is strongly stabilized in the presence of Ag-I, whereas a P-T pair is significantly destabilized. A direct correlation of oligonucleotide sequence, duplex stability prior to the addition of Ag-I, stabilization due to metal-mediated base pair formation, and performance of the molecular beacon was not observed. Nonetheless, the data show for the first time that metal-mediated base pairing can be applied in the detection of medicinally relevant SNPs.

引用

页码：3857 / 3861

页数：5

共 44 条

[31] 2,6-Bis(functionalized) purines as metal-ion-binding surrogate nucleobases that enhance hybridization with unmodified 2′-O-methyl oligoribonucleotides [J].