Generation of SMURF2 knockout human cells using the CRISPR/Cas9 system

被引：8

作者：

Ayyathan, Dhanoop Manikoth ^{[1
]}

Ilic, Nata ^{[1
]}

Gil-Henn, Hava ^{[2
]}

Blank, Michael ^{[1
]}

机构：

[1] Bar Ilan Univ, Fac Med Galilee, Lab Mol & Cellular Canc Biol, Safed, Israel

[2] Bar Ilan Univ, Fac Med Galilee, Cell Migrat & Invas Lab, Safed, Israel

来源：

ANALYTICAL BIOCHEMISTRY | 2017年 / 531卷

基金：

以色列科学基金会;

关键词：

SMURF2; CRISPR/Cas9; Knockout; Human cells; E3 UBIQUITIN LIGASES; STABILITY; CANCER;

D O I：

10.1016/j.ab.2017.05.024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The HECT domain E3 ubiquitin ligase SMURF2 regulates stability of several key protein targets involved in tumorigenesis, cell proliferation, migration, differentiation, and senescence. While altered levels and aberrant cellular distribution of SMURF2 were reported in different types of cancer, its role in tumorigenesis is far from understood. To elucidate the role of SMURF2 in cancer, appropriate human cancer cell models are needed. Here, we describe approaches that can be used to generate human normal and cancer cell strains knocked-out for SMURF2 using the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：56 / 59

页数：4

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