Anti-influenza A Virus Activity of Dendrobine and Its Mechanism of Action

被引：97

作者：

Li, Richan ^{[1
]}

Liu, Teng ^{[1
]}

Liu, Miaomiao ^{[1
]}

Chen, Feimin ^{[1
]}

Liu, Shuwen ^{[1
]}

Yang, Jie ^{[1
]}

机构：

[1] Southern Med Univ, Sch Pharmaceut Sci, Guangdong Prov Key Lab New Drug Screening, Guangzhou Key Lab Drug Res Emerging Virus Prevent, Guangzhou 510515, Guangdong, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2017年 / 65卷 / 18期

基金：

中国国家自然科学基金;

关键词：

Dendrobium nobile; dendrobine; antiviral; viral nucleoprotein; influenza A virus; NUCLEAR EXPORT; ANTIVIRAL ACTIVITY; INFLUENZA-VIRUSES; NUCLEOPROTEIN; IDENTIFICATION; NOBILE; PHENANTHRENES; INHIBITION; POLYMERASE; RESISTANCE;

D O I：

10.1021/acs.jafc.7b00276

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Dendrobine, a major component of Dendrobium nobile, increasingly draws attention for its wide applications in health care. Here we explore potential effects of dendrobine against influenza A virus and elucidate the underlying mechanism. Our results indicated that dendrobine possessed antiviral activity against influenza A viruses, including A/FM-1/1/47 (H1N1), A/Puerto Rico/8/34 H274Y (H1N1), and A/Aichi/2/68 (H3N2) with IC50 values of 3.39 +/- 0.32, 2.16 +/- 0.91, 5.32 +/- 1.68 mu g/mL, respectively. Mechanism studies revealed that dendrobine inhibited early steps in the viral replication cycle. Notably, dendrobine could bind to the highly conserved region of viral nucleoprotein (NP), subsequently restraining nuclear export of viral NP and its oligomerization. In conclusion, dendrobine shows potential to be developed as a promising agent to treat influenza virus infection. More importantly, the results provide invaluable information for the full application of the Traditional Chinese Medicine named "Shi Hu".

引用

页码：3665 / 3674

页数：10

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