Analysis of particle size distribution by particle tracking

被引:32
作者
Finder, C [1 ]
Wohlgemuth, M [1 ]
Mayer, C [1 ]
机构
[1] Univ Duisburg Gesamthsch, Inst Chem, D-47057 Duisburg, Germany
关键词
image analysis; microscopy; particle size; particle tracking; size distribution;
D O I
10.1002/ppsc.200400948
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Particle tracking is performed using a combination of dark field or fluorescence video microscopy with automatic image analysis. The optical detection together with the image analysis software allows for the time resolved localization of individual particles with diameters between 100 and 1000 nm. Observation of their Brownian motion over a set of time intervals leads to the determination of their mean square displacements under the given room temperature and viscosity. Hereby, the radii of a set of particles visible within a given optical frame are derived simultaneously. Rapid data analysis leads to reliable particle size histograms. The applicability of this method is demonstrated on polystyrene latices and PMMA nanospheres with radii between 51 nm and 202 nm.
引用
收藏
页码:372 / 378
页数:7
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