Separation of the enantiomers of ibuprofen and its major phase I metabolites in urine using capillary electrophoresis

被引：31

作者：

Bjornsdottir, I ^{[1
]}

Kepp, DR ^{[1
]}

Tjornelund, J ^{[1
]}

Hansen, SH ^{[1
]}

机构：

[1] Royal Danish Sch Pharm, Dept Analyt & Pharmaceut Chem, DK-2100 Copenhagen, Denmark

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 03期

关键词：

capillary electrophoresis; ibuprofen; chiral separation; dextrin; 10; heptakis (2,3,6-tri-O-methyl)-beta-cyclodextrin;

D O I：

10.1002/elps.1150190316

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A capillary electrophoresis method for determination of the enantiomers of ibuprofen and its major phase I metabolites: 2'-hydroxyibuprofen and 2'-carboxyibuprofen in urine samples have been developed. Cyclodextrins and linear dextrins have been investigated as chiral selectors. Simultaneous chiral separation of the enantiomers of ibuprofen, 2'-hydroxyibuprofen and 2'-carboxyibuprofen was obtained using a mixture of dextrin 10 and heptakis (2,3,6-tri-O-methyl)-beta-cyclodextrin in a 2-[N-morpholino]ethanesulphonic acid buffer, pH 5.26. The electroosmotic flow was reversed using hexadimethrine bromide as a buffer additive. The method can be used for the determination of the free enantiomers of ibuprofen, 2'-hydroxyibuprofen and 2'-carboxyibuprofen as well as for the indirect determination of their glucuronic acid conjugates in urine samples.

引用

页码：455 / 460

页数：6

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