The loading module of mycosubtilin: An adenylation domain with fatty acid selectivity

被引：66

作者：

Hansen, Darren B.

Bumpus, Stefanie B.

Aron, Zachary D.

Kelleher, Neil L.

Walsh, Christopher T.

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

[2] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 20期

关键词：

D O I：

10.1021/ja070890j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the cloning and characterization of the loading domain of mycosubtilin A synthetase responsible for the biosynthesis of mycosubtilin, a lipopeptide natural product from Bacillus subtilis. A truncated form of mycA was cloned and overexpressed and found to activate free fatty acids though an acyl-adenylate intermediate and loaded on the adjacent thiolation domain independently of coenzyme A, contradicting the literature proposal that the loading module is a coenzyme A ligase. The activation and loading of free fatty acids was characterized with a combination of traditional biochemical assays and electrospray ionization-Fourier transform mass spectrometry.

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页码：6366 / +

页数：3

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