Fabrication and characterization of biodegradable KH560 crosslinked chitin hydrogels with high toughness and good biocompatibility

Chitin hydrogels have multiple advantages of nontoxicity, biocompatibility, biodegradability, and three-dimensional hydrophilic polymer network structure similar to the macromolecular biological tissue. However, the mechanical strength of chitin hydrogels is relatively weak. Construction of chitin hydrogels with high me-chanical strength and good biocompatibility is essential for the successful applications in biomedical field. Herein, we developed double crosslinked chitin hydrogels by dissolving chitin in KOH/urea aqueous solution with freezing-thawing process, then using KH560 as cross-linking agent and coagulating in ethanol solution at low temperature. The obtained chitin/ KH560 (CK) hydrogels displayed good transparency and toughness with compressed nanofibrous network and porous structure woven with chitin nanofibers. Moreover, the optimal CK hydrogels exhibited excellent mechanical properties (sigma(b) = 1.92 +/- 0.21 Mpa; epsilon(b) = 71 +/- 5 %), high swelling ratio, excellent blood compatibility, biocompatibility and biodegradability, which fulfill the requirements of biomedical materials and showing potential applications in biomedicine.