Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant

被引：46

作者：

Mlcochova, Petra ^{[1
,2
]}

Collier, Dami ^{[1
,2
,3
]}

Ritchie, Allyson ^{[4
]}

Assennato, Sonny M. ^{[4
]}

Hosmillo, Myra ^{[5
]}

Goel, Neha ^{[4
]}

Meng, Bo ^{[1
,2
]}

Chatterjee, Krishna ^{[6
]}

Mendoza, Vivien ^{[6
]}

Temperton, Nigel ^{[7
]}

Kiss, Leo ^{[8
]}

James, Leo C. ^{[8
]}

Ciazynska, Katarzyna A. ^{[8
]}

Xiong, Xiaoli ^{[8
]}

Briggs, John A. G. ^{[8
]}

Nathan, James A. ^{[1
,2
]}

Mescia, Federica ^{[1
,2
]}

Bergamaschi, Laura ^{[1
,2
]}

Zhang, Hongyi ^{[9
]}

Barmpounakis, Petros ^{[10
]}

Demeris, Nikos ^{[10
,11
]}

Skells, Richard ^{[6
,11
]}

Lyons, Paul A. ^{[1
,2
]}

Bradley, John ^{[3
,12
]}

Baker, Steven ^{[1
,2
]}

Allain, Jean Pierre ^{[4
]}

Smith, Kenneth G. C. ^{[1
,2
]}

Bousfield, Rachel ^{[13
]}

Wilson, Michael ^{[13
]}

Sparkes, Dominic ^{[13
]}

Amoroso, Glenn ^{[14
]}

Gkrania-Klotsas, Effrosyni ^{[13
]}

Hardwick, Susie ^{[14
]}

Boyle, Adrian ^{[14
]}

Goodfellow, Ian ^{[5
]}

Gupta, Ravindra K. ^{[1
,2
,13
,15
]}

机构：

[1] Cambridge Inst Therapeut Immunol & Infect Dis CIT, Cambridge, England

[2] Univ Cambridge, Dept Med, Cambridge, England

[3] UCL, Div Infect & Immun, London WC1E 6BT, England

[4] Chesterford Res Pk, Diagnost Real World EU, Saffron Walden, Essex, England

[5] Univ Cambridge, Dept Pathol, Cambridge, England

[6] NIHR Cambridge Clin Res Facil, Cambridge, England

[7] Univ Kent, Medway Sch Pharm, Viral Pseudotype Unit, Canterbury, Kent, England

[8] Med Res Council Lab Mol Biol, Cambridge, England

[9] Cambridge Univ NHS Hosp Fdn Trust, Clin Microbiol & Publ Hlth Lab, Cambridge, England

[10] Athens Univ Econ & Business, Dept Stat, Athens, Greece

[11] Univ Cambridge, Cambridge Clin Trials Unit Canc Theme, Cambridge, England

[12] Cambridge Biomed Res Ctr, Natl Inst Hlth Res, Cambridge, England

[13] Cambridge Univ NHS Hosp Fdn Trust, Dept Infect Dis, Cambridge, England

[14] Cambridge Univ NHS Hosp Fdn Trust, Dept Emergency Med, Cambridge, England

[15] Africa Hlth Res Inst, Durban, South Africa

来源：

CELL REPORTS MEDICINE | 2020年 / 1卷 / 06期

基金：

英国医学研究理事会; 英国惠康基金; 欧洲研究理事会;

关键词：

COVID-19; D614G; point of care testing; rapid diagnoses; SARS-CoV-2;

D O I：

10.1016/j.xcrm.2020.100099

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%-50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to detect anti-spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to either wildtype or the D614G spike mutant SARS-CoV-2. The overall detection rate for COVID-19 is 79.2% (95% CI 57.8-92.9) by rapid NAAT alone. The combined point of care antibody test and rapid NAAT is not affected by D614G and results in very high sensitivity for COVID-19 diagnosis with very high specificity.

引用

页数：14

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