Effect of temperature in reversed phase liquid chromatography

被引:200
作者
Guillarme, D [1 ]
Heinisch, S [1 ]
Rocca, JL [1 ]
机构
[1] Univ Lyon 1, CNRS, UMR 5180, Analyt Sci Lab, F-69622 Villeurbanne, France
关键词
high temperature liquid chromatography; kinetic and thermodynamic behavior; temperature control;
D O I
10.1016/j.chroma.2004.08.052
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The high temperature liquid chromatography (HTLC) reveals interesting chromatographic properties but even now, it misses some theoretical aspects concerning the influence of high temperature on thermodynamic and kinetic aspects of chromatography: such a knowledge is very essential for method development. In this work, the effect of temperature on solute behavior has been studied using various stationary phases which are representative of the available thermally stable materials present on the market. The thermodynamic properties were evaluated by using different mobile phases: acetonitrile-water, methanol-water and pure water. The obtained results were discussed on the basis of both type of mobile phases and type of stationary phases. Type of mobile phase was found to play an important role on the retention of solutes. The kinetic aspect was studied at various temperatures ranging from ambient temperature to high temperature (typically from about 30 to 200degreesC) by fitting the experimental data with the Knox equation and it was shown that the efficiency is improved significantly when the temperature is increased. In this paper, we also discussed the problem of temperature control for thermostating columns which may represent a significant source of peak broadening: by taking into account the three main parameters such as heat transfer, pressure drop and band broadening resulting from the preheating tube, suitable rules are set up for a judicious choice of the column internal diameter. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:39 / 51
页数:13
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