Observations of the Mechanisms of Electromediated DNA Uptake - From Vesicles to Tissues

被引：23

作者：

Golzio, Muriel ^{[1
,2
]}

Escoffre, Jean-Michel ^{[1
,2
]}

Portet, Thomas ^{[1
,2
,3
]}

Mauroy, Chloe ^{[1
,2
]}

Teissie, Justin ^{[1
,2
]}

Dean, David S. ^{[3
]}

Rols, Marie-Pierre ^{[1
,2
]}

机构：

[1] CNRS, IPBS, UMR 5089, F-31077 Toulouse, France

[2] Univ Toulouse, IPBS, UPS, F-31077 Toulouse, France

[3] Univ Toulouse 3, CNRS, UMR 5152, Phys Theor Lab, F-31062 Toulouse, France

来源：

CURRENT GENE THERAPY | 2010年 / 10卷 / 04期

关键词：

Delivery; electric field; electroporation; gene transfer; membrane; microscopy; IN-VIVO ELECTROPORATION; MEDIATED GENE-TRANSFER; ELECTRIC-FIELD DISTRIBUTION; MAMMALIAN-CELLS; SKELETAL-MUSCLE; PLASMID DNA; NAKED DNA; GIANT VESICLES; MEMBRANE PERMEABILIZATION; STRETCHED VESICLES;

D O I：

10.2174/156652310791823461

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We discuss experimental observations of DNA uptake induced by electropermeabilisation. First we describe how experiments on giant unilamellar vesicles can be used to understand the effect of electric fields on lipid membranes and the associated transfer of DNA across the plasma membrane. We then discuss how DNA interacts with electropermeabilised cells in culture and how gene expression is correlated to observations of interactions between DNA and the cell membrane. Finally we discuss how DNA uptake occurs and can be optimised for the most medically relevant case of tissues.

引用

页码：256 / 266

页数：11

共 117 条

[31] Direct visualization at the single-cell level of electrically mediated gene delivery [J].