Determination of the metabolites of gestrinone in human urine by high performance liquid chromatography, liquid chromatography/mass spectrometry and gas chromatography/mass spectrometry

被引：0

作者：

Kim, Y

Lee, Y

Kim, M

Yim, YH

Lee, W

机构：

[1] Korea Inst Sci & Technol, Doping Control Ctr, Seoul, South Korea

[2] LG Chem Ltd, Taejon, South Korea

[3] Kyung Hee Univ, Res Inst Basic Sci, Seoul, South Korea

[4] Kyung Hee Univ, Dept Chem, Seoul, South Korea

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2000年 / 14卷 / 18期

关键词：

Ether derivatives - Fragment ions - Gaschromatography-mass spectrometry - High-performance liquid chromatography - Human urine - LC-MS-MS - Liquid chromatography - mass spectrometries - Parent ions - Trimethylsilyl - Trimethylsilyl ethers;

D O I：

10.1002/1097-0231(20000930)14:18<1717::AID-RCM65>3.0.CO;2-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gestrinone was studied by high performance liquid chromatography (HPLC) for screening and by gas chromatography/mass spectrometry (GC/MS) for confirmation. When the chromatograms of blank, spiked urine and dosed urine were compared by HPLC, two unknown metabolites were found and these were excreted as the conjugated forms. Metabolites 1 and 2 were tested by LC/MS and LC/MS/MS and both had parent ions at m/z 325, The fragment ion of metabolite 1 was at m/z 263 and ions for metabolite 2 were m/z 307 [MH - H2O](+), 289, 279 and 241. LC/MS/MS of m/z 263 as the parent ion of metabolite 1 gave fragment ions at m/z 245 and 217, which were assumed to be [263 - H2O](+) and [235 - H2O](+), respectively. The trimethylsilyl (TMS)-enol-TMS ether derivative of gestrinone displayed three peaks in its GC/MS chromatogram, formed by tautomerism. Copyright (C) 2000 John Wiley gr Sons, Ltd.

引用

页码：1717 / 1726

页数：10

共 8 条

[1] THE ANALYSIS OF TRENBOLONE AND THE HUMAN URINARY METABOLITES OF TRENBOLONE ACETATE BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY AND GAS-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY [J].