Proteomics of extreme freezing tolerance in Siberian spruce (Picea obovata)

被引：44

作者：

Kjellsen, Trygve D. ^{[1
]}

Shiryaeva, Liudmila ^{[2
]}

Schroder, Wolfgang P. ^{[2
]}

Strimbeck, G. Richard ^{[1
]}

机构：

[1] Norwegian Univ Sci & Technol, Dept Biol, N-7491 Trondheim, Norway

[2] Umea Univ, Dept Chem, SE-90187 Umea, Sweden

来源：

JOURNAL OF PROTEOMICS | 2010年 / 73卷 / 05期

基金：

瑞典研究理事会;

关键词：

Freezing tolerance; Picea obovata; DIGE; MALDI-TOF/TOF; OPLS-DA; Dehydrins; BETULA-PUBESCENS EHRH; COLD-ACCLIMATION; LOW-TEMPERATURE; PLASMA-MEMBRANE; SEASONAL-CHANGES; ATPASE ACTIVITY; BARK TISSUES; PROTEIN; DEHYDRINS; STRESS;

D O I：

10.1016/j.jprot.2009.12.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Differential expression of proteins in needles of the extreme freeze tolerant conifer Picea obovata during September, October and November was analyzed using DIGE technology and multivariate analysis More than 1200 spots were detected, and the abundance of 252 of these spots was significantly altered during the course of acclimation The 252 spots were clustered into five distinct expression profiles Among the protein spots showing differential expression, 43 were identified by MALDI-TOF/TOF and twelve of them matched proteins associated with various biotic and abiotic stress responses in other plants Dehydrins, Hsp70s, AAA(+) ATPases, lipocalin, cyclophilins, glycine-rich protein (GNP) and several reactive oxygen intermediate scavenging proteins showed increased accumulation levels from September to November The expression profiles and putative role of the identified proteins during acclimation and freezing tolerance are discussed (C) 2010 Elsevier B V All rights reserved

引用

页码：965 / 975

页数：11

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