Quantifying the relative amount of mouse and human DNA in cancer xenografts using species-specific variation in gene length

被引：31

作者：

Lin, Ming-Tseh ^{[1
]}

Tseng, Li-Hui ^{[1
,2
]}

Kamiyama, Hirohiko ^{[1
]}

Kamiyama, Mihoko ^{[1
]}

Lim, Phillip ^{[1
]}

Hidalgo, Manuel ^{[4
]}

Wheelan, Sarah ^{[3
]}

Eshleman, James R. ^{[1
,4
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Pathol, Johns Hopkins Hosp, Baltimore, MD 21205 USA

[2] Natl Taiwan Univ Hosp, Dept Med Genet, Taipei, Taiwan

[3] Johns Hopkins Univ, Sch Med, Dept Oncol Biostat & Bioinformat, Johns Hopkins Hosp, Baltimore, MD 21205 USA

[4] Johns Hopkins Univ, Sch Med, Dept Oncol, Johns Hopkins Hosp, Baltimore, MD 21205 USA

来源：

BIOTECHNIQUES | 2010年 / 48卷 / 03期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

xenograft; quantification; species-specific length variation; chimerism; capillary gel electrophoresis; COMPARATIVE GENOMIC HYBRIDIZATION; MARROW TRANSPLANT ENGRAFTMENT; QUANTITATIVE-DETERMINATION; CHROMOSOMAL IMBALANCES; ADENOCARCINOMA; TUMORS; ENRICHMENT; AMELOGENIN; PATHWAYS; PANCREAS;

D O I：

10.2144/000113363

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human cancer cell lines and xenografts are valuable samples for whole-genome sequencing of human cancer. Tumors can be maintained by serial xenografting in athymic (nude) or severe combined immunodeficient (SCID) mice. In the current study, we developed a molecular assay to quantify the relative contributions of human and mouse in mixed DNA samples. The assay was designed based on deletion/insertion variation between human and mouse genomes. The percentage of mouse DNA was calculated according to the relative peak heights of PCR products analyzed by capillary electrophoresis. Three markers from chromosomes 9 and 10 accurately predicted the mouse genome ratio and were combined into a multiplex PCR reaction. We used the assay to quantify the relative DNA amounts of 93 mouse xenografts used for a recently reported integrated genomic analysis of human pancreatic cancer. Of the 93 xenografts, the mean percentage of contaminating mouse DNA was 47%, ranging from 17% to 73%, with 43% of samples having >50% mouse DNA. We then comprehensively compared the human and mouse genomes to identify 370 additional candidate gene loci demonstrating human-mouse length variation. With increasing whole-genome sequencing of human cancers, this assay should be useful to monitor strategies to enrich human cancer cells from mixed human-mouse cell xenografts. Finally, we discuss how contaminating mouse DNA affects next-generation DNA sequencing.

引用

页码：211 / 218

页数：6

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