Hydrogen exchange studies of protein structure

被引：81
作者：
Raschke, TM [1 ]
Marqusee, S [1 ]
机构：
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
来源：
CURRENT OPINION IN BIOTECHNOLOGY | 1998年 / 9卷 / 01期
基金：
美国国家科学基金会;
关键词：
D O I：
10.1016/S0958-1669(98)80088-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Hydrogen exchange techniques, with their residue-level specificity, exquisite sensitivity, and adaptability to many solution conditions, are becoming essential to the study of protein stability, folding and dynamics. Recent studies have elucidated the structures of intermediates formed transiently during protein folding and rare partially folded ensembles present at equilibrium. Analysis of hydrogen exchange mechanisms has revealed protein stability and kinetics at the level of individual residues. (C) Current Biology Ltd ISSN 0958-1669.
引用
收藏
页码：80 / 86
页数：7
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