Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice

被引：9

作者：

Adhikari, Nirpesh ^{[1
]}

Neupane, Sanjiv ^{[1
]}

Aryal, Yam Prasad ^{[1
]}

Choi, Mi ^{[2
]}

Sohn, Wern-Joo ^{[3
]}

Lee, Youngkyun ^{[1
]}

Jung, Jae-Kwang ^{[4
]}

Ha, Jung-Hong ^{[5
]}

Choi, So-Young ^{[6
]}

Suh, Jo-Young ^{[7
]}

Kim, Ji-Youn ^{[8
]}

Rho, Mun-Chual ^{[9
]}

Lee, Tae-Hoon ^{[10
]}

Yamamoto, Hitoshi ^{[11
]}

An, Chang-Hyeon ^{[2
]}

Kim, Sang-Hyun ^{[12
]}

An, Seo-Young ^{[2
]}

Kim, Jae-Young ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Sch Dent, Dept Biochem, IHBR, Daegu, South Korea

[2] Kyungpook Natl Univ, Sch Dent, Dept Oral & Maxillofacial Radiol, IHBR, Daegu, South Korea

[3] Daegu Haany Univ, Premajor Cosmet & Pharmaceut, Gyongsan, South Korea

[4] Kyungpook Natl Univ, Sch Dent, Dept Oral Med, IHBR, Daegu, South Korea

[5] Kyungpook Natl Univ, Sch Dent, Dept Conservat Dent, IHBR, Daegu, South Korea

[6] Kyungpook Natl Univ, Sch Dent, Dept Oral & Maxillofacial Surg, IHBR, Daegu, South Korea

[7] Kyungpook Natl Univ, Sch Dent, Dept Periodontol, IHBR, Daegu, South Korea

[8] Gachon Univ, Dept Dent Hyg, Coll Hlth Sci, Incheon, South Korea

[9] Korea Res Inst Biosci & Biotechnol, Immunoregulatory Mat Res Ctr, Jeongeup Si, South Korea

[10] Chonnam Natl Univ, Sch Dent, Dept Oral Biochem, Dent Sci Res Inst,Med Res Ctr Biomineralizat Diso, Gwangju, South Korea

[11] Tokyo Dent Coll, Dept Histol & Dev Biol, Tokyo, Japan

[12] Kyungpook Natl Univ, Sch Med, Dept Pharmacol, Daegu, South Korea

来源：

JOURNAL OF PERIODONTAL RESEARCH | 2019年 / 54卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

alveolar bone; chronic periodontitis; inflammation; regeneration; INHIBITS OSTEOCLAST DIFFERENTIATION; LIGATURE-INDUCED PERIODONTITIS; LIGAMENT; INFLAMMATION; OSTEOBLAST; ARTHRITIS; PATHWAYS;

D O I：

10.1111/jre.12657

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objective We evaluated the role of oleanolic acid acetate (OAA), a triterpenoid commonly used in the treatment of liver disorders, inflammatory diseases, and metastasis, in bone formation after tooth loss by periodontitis. Background Periodontitis causes the sequential degradation of the alveolar bone and associated structures, resulting in tooth loss. Several studies have attempted to regenerate the bone for implantation following tooth loss. Methods Maxillary left second molar was extracted from 8-week-old male mice following induction of periodontitis by ligature for 5 days. The extraction socket was treated with 50 ng/mu L OAA for 1, 2, and 3 weeks. Detailed morphological changes were examined using Masson's trichrome staining, and the precise localization patterns of various signaling molecules, including CD31, F4/80, interleukin (IL)-6, and osteocalcin, were observed. The volume of bone formation was examined by Micro-CT. Osteoclasts were enumerated using tartrate-resistant acid phosphatase (TRAP) staining. For molecular dissection of signaling molecules, we employed the hanging-drop in vitro cultivation method at E14 for 1 day and examined the expression pattern of transforming growth factor (TGF)-beta superfamily and Wnt signaling genes. Results Histomorphometrical examinations showed facilitated bone formation in the extraction socket following OAA treatment. In addition, OAA-treated specimens showed the altered localization patterns of inflammatory and bone formation-related signaling molecules including CD31, F4/80, IL-6, and osteocalcin. Also, embryonic tooth germ mesenchymal tissue cultivation with OAA treatment showed the significant altered expression patterns of signaling molecules such as transforming growth factor (TGF)-beta superfamily and Wnt signaling. Conclusions Oleanolic acid acetate induces bone formation and remodeling through proper modulation of osteoblast, osteoclast, and inflammation with regulations of TGF-beta and Wnt signaling.

引用

页码：533 / 545

页数：13

共 45 条

[41] TGF-β and BMP signaling in osteoblast, skeletal development, and bone formation, homeostasis and disease [J].