Single-particle cryo-EM data acquisition by using direct electron detection camera

被引：41

作者：

Wu, Shenping ^{[1
]}

Armache, Jean-Paul ^{[1
]}

Cheng, Yifan ^{[1
,2
]}

机构：

[1] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA

[2] Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94158 USA

来源：

MICROSCOPY | 2016年 / 65卷 / 01期

基金：

美国国家卫生研究院;

关键词：

electron cryo-microscopy; single-particle cryo-EM; direct electron detection camera; BEAM-INDUCED MOTION; ATOMIC-RESOLUTION; ANISOTROPIC MAGNIFICATION; CRYOELECTRON MICROSCOPY; ANGSTROM RESOLUTION; CRYOMICROSCOPY; SPECIMEN; COMPLEX; IMAGES;

D O I：

10.1093/jmicro/dfv355

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Recent advances in single-particle electron cryo-microscopy (cryo-EM) were largely facilitated by the application of direct electron detection cameras. These cameras feature not only a significant improvement in detective quantum efficiency but also a high frame rate that enables images to be acquired as 'movies' made of stacks of many frames. In this review, we discuss how the applications of direct electron detection cameras in cryo-EM have changed the way the data are acquired.

引用

页码：35 / 41

页数：7

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