Tissue immunoblot procedure for detection of Neotyphodium coenophialum in tall fescue - Optimization of time requirements

被引:0
作者
Hiatt, EE [1 ]
Hill, NS [1 ]
Bouton, JH [1 ]
机构
[1] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30601 USA
来源
NEOTYPHODIUM/GRASS INTERACTIONS | 1997年
关键词
D O I
暂无
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Published tissue immunoblot protocols for detecting Neotyphodium coenophialum are for polyclonal antibodies (Gwinn et al., 1991; An et al. 1993) and require approximately 24 h to conduct. Commercial acceptance of immunoblot technology may be more widespread if the protocol could be shortened to accommodate labor costs and/or scheduling. The objective of this experiment was to evaluate the time requirements of the tissue immunoblot procedure and determine the minimum amount of time required to give accurate and consistent results.
引用
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页码:265 / 266
页数:2
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