Tissue immunoblot procedure for detection of Neotyphodium coenophialum in tall fescue - Optimization of time requirements

被引:0
作者
Hiatt, EE [1 ]
Hill, NS [1 ]
Bouton, JH [1 ]
机构
[1] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30601 USA
来源
NEOTYPHODIUM/GRASS INTERACTIONS | 1997年
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暂无
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Published tissue immunoblot protocols for detecting Neotyphodium coenophialum are for polyclonal antibodies (Gwinn et al., 1991; An et al. 1993) and require approximately 24 h to conduct. Commercial acceptance of immunoblot technology may be more widespread if the protocol could be shortened to accommodate labor costs and/or scheduling. The objective of this experiment was to evaluate the time requirements of the tissue immunoblot procedure and determine the minimum amount of time required to give accurate and consistent results.
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页码:265 / 266
页数:2
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