Tissue immunoblot procedure for detection of Neotyphodium coenophialum in tall fescue - Optimization of time requirements

被引：0

作者：

Hiatt, EE ^{[1
]}

Hill, NS ^{[1
]}

Bouton, JH ^{[1
]}

机构：

[1] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30601 USA

来源：

NEOTYPHODIUM/GRASS INTERACTIONS | 1997年

关键词：

D O I：

暂无

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Published tissue immunoblot protocols for detecting Neotyphodium coenophialum are for polyclonal antibodies (Gwinn et al., 1991; An et al. 1993) and require approximately 24 h to conduct. Commercial acceptance of immunoblot technology may be more widespread if the protocol could be shortened to accommodate labor costs and/or scheduling. The objective of this experiment was to evaluate the time requirements of the tissue immunoblot procedure and determine the minimum amount of time required to give accurate and consistent results.

引用

页码：265 / 266

页数：2

共 50 条

[11] Neotyphodium coenophialum mycelial protein and herbage mass effects on ergot alkaloid concentration in tall fescue [J].

Hiatt, EE ;

Hill, NS .

NEOTYPHODIUM/GRASS INTERACTIONS, 1997, :257-259

[12] Occurrence of the fungal endophyte Neotyphodium coenophialum in leaf blades of tall fescue and implications for stock health [J].

Christensen, MJ ;

Easton, HS ;

Simpson, WR ;

Tapper, BA .

NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH, 1998, 41 (04) :595-602

[13] Use of a rat model to evaluate tall fescue seed infected with introduced strains of Neotyphodium coenophialum [J].

Roberts, CA ;

Spiers, DE ;

Karr, AL ;

Benedict, HR ;

Sleper, DA ;

Eichen, PA ;

West, CP ;

Piper, EL ;

Rottinghaus, GE .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (20) :5742-5745

[14] Modification of rhizosphere activity of tall fescue by the fungal endophyte Neotyphodium coenophialum and consequences for mineral uptake [J].

Malinowski, DP ;

Belesky, DP ;

Brauer, DK ;

Alloush, GA .

1998 AMERICAN FORAGE AND GRASSLAND COUNCIL, PROCEEDINGS: FORAGES AT THE CROSSROADS, 1998, 7 :176-180

[15] The endophyte Neotyphodium coenophialum affects root morphology of tall fescue grown under phosphorus deficiency [J].

Malinowski, DP ;

Brauer, DK ;

Belesky, DP .

JOURNAL OF AGRONOMY AND CROP SCIENCE, 1999, 183 (01) :53-60

[16] NEOTYPHODIUM COENOPHIALUM-INFECTED TALL FESCUE AND ITS POTENTIAL APPLICATION IN THE PHYTOREMEDIATION OF SALINE SOILS [J].

Yin, L. ;

Ren, A. ;

Wei, M. ;

Wu, L. ;

Zhou, Y. ;

Li, X. ;

Gao, Y. .

INTERNATIONAL JOURNAL OF PHYTOREMEDIATION, 2014, 16 (03) :235-246

[17] A POLYMERASE CHAIN REACTION-BASED PROCEDURE FOR DETECTION OF ACREMONIUM-COENOPHIALUM IN TALL FESCUE [J].

DOSS, RP ;

WELTY, RE .

PHYTOPATHOLOGY, 1995, 85 (08) :913-917

[18] Neotyphodium coenophialum mycelial protein and herbage mass effects on ergot alkaloid concentration in tall fescue [J].

Hiatt, EE ;

Hill, NS .

JOURNAL OF CHEMICAL ECOLOGY, 1997, 23 (12) :2721-2736

[19] Neotyphodium coenophialum Mycelial Protein and Herbage Mass Effects on Ergot Alkaloid Concentration in Tall Fescue [J].

E. E. Hiatt ;

N. S. Hill .

Journal of Chemical Ecology, 1997, 23 :2721-2736

[20] Changes in Neotyphodium coenophialum infestation levels in tall fescue pastures due to different grazing pressures [J].

Gwinn, KD ;

Fribourg, HA ;

Waller, JC ;

Saxton, AM ;

Smith, MC .

CROP SCIENCE, 1998, 38 (01) :201-204

← 1 2 3 4 5 →