Tissue immunoblot procedure for detection of Neotyphodium coenophialum in tall fescue - Optimization of time requirements

Published tissue immunoblot protocols for detecting Neotyphodium coenophialum are for polyclonal antibodies (Gwinn et al., 1991; An et al. 1993) and require approximately 24 h to conduct. Commercial acceptance of immunoblot technology may be more widespread if the protocol could be shortened to accommodate labor costs and/or scheduling. The objective of this experiment was to evaluate the time requirements of the tissue immunoblot procedure and determine the minimum amount of time required to give accurate and consistent results.