Developmentally regulated activation of a SINE B2 repeat as a domain boundary in organogenesis

被引：206
作者：
Lunyak, Victoria V.
Prefontaine, Gratien G.
Nunez, Esperanza
Cramer, Thorsten
Ju, Bong-Gun
Ohgi, Kenneth A.
Hutt, Kasey
Roy, Rosa
Garcia-Diaz, Angel
Zhu, Xiaoyan
Yung, Yun
Montoliu, Lluis
Glass, Christopher K.
Rosenfeld, Michael G.
机构：
[1] Univ Calif San Diego, Sch Med, Howard Hughes Med Inst, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Dept Med, Div Endocrinol, La Jolla, CA 92093 USA
[3] Univ Calif San Diego, Dept Cellular & Mol Med, Dept Med, Sch Med, La Jolla, CA 92093 USA
[4] CSIC, CNB, Dept Mol & Cellular Biol, Madrid 28049, Spain
[5] Charite Univ Med Berlin, Dept Gastroenterol & Hepatol, D-13353 Berlin, Germany
来源：
SCIENCE | 2007年 / 317卷 / 5835期
关键词：
D O I：
10.1126/science.1140871
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The temporal and spatial regulation of gene expression in mammalian development is linked to the establishment of functional chromatin domains. Here, we report that tissue-specific transcription of a retrotransposon repeat in the murine growth hormone locus is required for gene activation. This repeat serves as a boundary to block the influence of repressive chromatin modifications. The repeat element is able to generate short, overlapping Pol II- and Pol III-driven transcripts, both of which are necessary and sufficient to enable a restructuring of the regulated locus into nuclear compartments. These data suggest that transcription of interspersed repetitive sequences may represent a developmental strategy for the establishment of functionally distinct domains within the mammalian genome to control gene activation.
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页码：248 / 251
页数：4
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