Cyperus rotundus extract inhibits acetylcholinesterase activity from animal and plants as well as inhibits germination and seedling growth in wheat and tomato

被引:42
作者
Sharma, Rashmi [1 ]
Gupta, Rajendra [1 ]
机构
[1] Univ Delhi, Dept Bot, Delhi 110007, India
关键词
acetylcholinesterase; allelopathy; anticholinesterases; Cyperus rotundus; nutgrass; tomato; wheat;
D O I
10.1016/j.lfs.2007.01.060
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Cyperus rotundus (nutgrass) is the world's worst invasive weed through tubers. Its success in dominating natural habitats depends on its ability to prevent herbivory, and to kill or suppress other plants growing in its vicinity. The present study was done to investigate whether chemicals in nutgrass target neuronal and non-neuronal acetylcholinesterases to affect surrounding animals and plants respectively. Methanolic extract of tubers of nutgrass strongly inhibited activity of AChE from electric eel, wheat and tomato. It also inhibited seed germination and seedling growth in wheat and tomato. Our results suggest that inhibitor of AChE in nutgrass possibly acts as agent of plant's war against (a) herbivore animals, and (b) other plants trying to grow in the same habitat. An antiAChE from nutgrass has been purified by employing chromatography and crystallization. The structural determination of the purified inhibitor is in progress. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:2389 / 2392
页数:4
相关论文
共 18 条
[1]  
[Anonymous], WORLDS WORST WEEDS D
[2]   Allelopathy and exotic plant invasion: From molecules and genes to species interactions [J].
Bais, HP ;
Vepachedu, R ;
Gilroy, S ;
Callaway, RM ;
Vivanco, JM .
SCIENCE, 2003, 301 (5638) :1377-1380
[3]   A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].
ELLMAN, GL ;
COURTNEY, KD ;
ANDRES, V ;
FEATHERSTONE, RM .
BIOCHEMICAL PHARMACOLOGY, 1961, 7 (02) :88-&
[4]   Tissue distribution of cholinesterases and anticholinesterases in native and transgenic tomato plants [J].
Fletcher, SP ;
Geyer, BC ;
Smith, A ;
Evron, T ;
Joshi, L ;
Soreq, H ;
Mor, TS .
PLANT MOLECULAR BIOLOGY, 2004, 55 (01) :33-43
[5]   A survey of plants for presence of cholinesterase activity [J].
Gupta, A ;
Gupta, R .
PHYTOCHEMISTRY, 1997, 46 (05) :827-831
[6]  
GUPTA MB, 1971, INDIAN J MED RES, V59, P76
[7]  
Hamada T, 1993, Yakushigaku Zasshi, V28, P63
[8]   Ecophysiological aspects of allelopathy [J].
Inderjit ;
Duke, SO .
PLANTA, 2003, 217 (04) :529-539
[9]   Rotundines A-C, three novel sesquiterpene alkaloids from Cyperus rotundus [J].
Jeong, SJ ;
Miyamoto, T ;
Inagaki, M ;
Kim, YC ;
Higuchi, R .
JOURNAL OF NATURAL PRODUCTS, 2000, 63 (05) :673-675
[10]   Pharmacokinetic and pharmacological interactions between ticlopidine hydrochloride and Kangen-Karyu -: Chinese traditional herbal medicine [J].
Makino, T ;
Wakushima, H ;
Okamoto, T ;
Okukubo, Y ;
Deguchi, Y ;
Kano, Y .
PHYTOTHERAPY RESEARCH, 2003, 17 (09) :1021-1024