The Key Role of c-Fos for Immune Regulation and Bacterial Dissemination in Brucella Infected Macrophage

被引：48

作者：

Hop, Huynh T. ^{[1
]}

Arayan, Lauren T. ^{[1
]}

Huy, Tran X. N. ^{[1
]}

Reyes, Alisha W. B. ^{[1
]}

Vu, Son H. ^{[1
]}

Min, Wongi ^{[1
]}

Lee, Hu J. ^{[1
]}

Rhee, Man H. ^{[2
]}

Chang, Hong H. ^{[3
]}

Kim, Suk ^{[1
,3
]}

机构：

[1] Gyeongsang Natl Univ, Inst Anim Med, Coll Vet Med, Jinju, South Korea

[2] Kyungpook Natl Univ, Coll Vet Med, Daegu, South Korea

[3] Gyeongsang Natl Univ, Inst Agr & Life Sci, Jinju, South Korea

来源：

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | 2018年 / 8卷

关键词：

Brucella abortus; c-Fos; MAPKs; TLR-4; IL-10; MYCOBACTERIUM-TUBERCULOSIS; INTRACELLULAR REPLICATION; INFLAMMATORY RESPONSE; CELL-PROLIFERATION; FLOW-CYTOMETRY; ABORTUS; EXPRESSION; APOPTOSIS; DEATH; MICE;

D O I：

10.3389/fcimb.2018.00287

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The cellular oncogene c-Fos (c-Fos) is a component of activator protein 1 (AP1), a master transcriptional regulator of cells. The suppression of c-Fos signaling by siRNA treatment resulted in significant induction of TLR4, which subsequently activates p38 and ERK1/2 mitogen-activated protein kinases (MAPKs) and enhances F-actin polymerization, leading to an increase in B. abortus phagocytosis. During B. abortus infection, c-Fos signaling is induced, which activates the downstream innate-immunity signaling cascade for bacterial clearance. The inhibition of c-Fos signaling led to increased production of interleukin 10 (IL-10), which partially suppressed lysosome-mediated killing, resulting in increased survival of B. abortus inside macrophages. We present evidence of the regulatory role played by the c-Fos pathway in proliferation during B. abortus infection; however, this was independent of the anti-Brucella effect of this pathway. Another finding is the essential contribution of c-Fos/TRAIL to infected-cell necrosis, which is a key event in bacterial dissemination. These data provide the mechanism via which c-Fos participates in host defense mechanisms against Brucella infection and in bacterial dissemination by macrophages.

引用

页数：16

共 51 条

[1] Toll-like receptor 4 expression is required to control chronic Mycobacterium tuberculosis infection in mice [J].