Electrochemical Detection of Human Interleukin-15 using a Graphene Oxide-Modified Screen-Printed Carbon Electrode

被引：9
作者：
Norfun, Poachanee
Suree, Nuttee
Kungwan, Nawee
Punyodom, Winita
Jakmunee, Jaroon
Ounnunkad, Kontad [1 ,2 ]
机构：
[1] Chiang Mai Univ, Fac Sci, Dept Chem, Chiang Mai 50200, Thailand
[2] Chiang Mai Univ, Fac Sci, Ctr Excellence Innovat Chem PERCH CIC, Chiang Mai 50200, Thailand
来源：
ANALYTICAL LETTERS | 2017年 / 50卷 / 07期
关键词：
Biomarkers; early HIV-1 infection; electrochemical detection; graphene oxide; immunosensor; interleukin-15; screen-printed carbon electrode; LABEL-FREE IMMUNOSENSOR; HUMAN-IMMUNOGLOBULIN G; GOLD NANOPARTICLES; AU NANOPARTICLES; CANCER BIOMARKER; ANTIBODY; IMMUNOASSAY; DISPERSION; RESPONSES; IL-15;
D O I：
10.1080/00032719.2016.1216123
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Biofunctionalizing a simple and disposable graphene oxide-modified screen-printed carbon electrode with anti-interleukin-15 antibodies has been successfully demonstrated for the first time for the label-free electrochemical detection of interleukin-15, a biomarker of early HIV infection. To improve the electrochemical reactivity and introduce carboxylic groups on the surface of screen-printed carbon electrode, high-quality graphene oxide was used for the modification of screen-printed carbon electrode. With simple modification of the screen-printed carbon electrode, the device exhibited satisfactory sensitivity, selectivity, stability, reproducibility, and regenerability. The immunosensor provided a detection limit of 3.51ngmL(-1) and a sensitivity of 0.5655 mu A cm(-2)mLng(-1). The simply constructed immunosensor thus rendered promising device for immunoreactions on the surface of the electrode.
引用
收藏
页码：1112 / 1125
页数：14
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