Crystallization and preliminary X-ray analysis of cecropin B from Bombyx mori

被引：2

作者：

Liu, Zhongyuan ^{[2
,3
]}

Zhou, Qiangjun ^{[4
]}

Mao, Xinfang ^{[3
]}

Zheng, Xiangdong ^{[2
]}

Guo, Jiubiao ^{[2
]}

Zhang, Fuchun ^{[3
]}

Wen, Tingyi ^{[1
]}

Pang, Hai ^{[2
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, Dept Ind Microbiol & Biotechnol, Beijing 100101, Peoples R China

[2] Tsinghua Univ, Sch Med, Beijing 100084, Peoples R China

[3] Xinjiang Univ, Xinjiang Key Lab Biol Resources & Genet Engn, Urumqi 830046, Peoples R China

[4] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2010年 / 66卷

基金：

中国国家自然科学基金;

关键词：

HELICAL ANTIMICROBIAL PEPTIDES; ORIENTED LIPID-BILAYERS; PORE-FORMING PEPTIDE; SOLID-STATE NMR; INSECT IMMUNITY; INNATE IMMUNITY; CONFORMATION; SPECTROSCOPY; ORIENTATION; MECHANISM;

D O I：

10.1107/S1744309110020130

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cecropin B is a 37-residue cationic antimicrobial peptide derived from the haemolymph of Bombyx mori. The precise mechanism by which cecropins exert their antimicrobial and cytolytic activities is not well understood. Crystals of cecropin B were obtained by the hanging-drop vapour-diffusion method using polyethylene glycol as a precipitant at 289 K. The crystal diffracted to 1.43 angstrom resolution using X-ray radiation and belonged to the orthorhombic space group P1, with unit-cell parameters a = 15.08, b = 22.75, c = 30.20 angstrom, alpha = 96.9, beta = 103.1, gamma = 96.5 degrees. The asymmetric unit contained only one molecule of cecropin B, with a calculated Matthews coefficient of 2.48 angstrom(3) Da(-1) and a solvent content of 50.4%.

引用

页码：851 / 853

页数：3

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