K562 cell-derived exosomes suppress the adhesive function of bone marrow mesenchymal stem cells via delivery of miR-711

被引：14

作者：

Jiang, Yu-Huan ^{[1
]}

Liu, Jing ^{[1
]}

Lin, Jin ^{[1
]}

Li, Shu-Qi ^{[1
]}

Xu, Yan-Mei ^{[1
]}

Min, Qing-Hua ^{[1
]}

Zhong, Qiong-Hui ^{[1
]}

Sun, Fan ^{[1
]}

Li, Jing ^{[2
]}

You, Xia-Hong ^{[1
]}

Liao, Kai-Li ^{[1
]}

Qin, Ting-Yu ^{[1
]}

Zhao, Cui ^{[1
]}

Huang, Bo ^{[1
]}

Wang, Xiao-Zhong ^{[1
]}

机构：

[1] Nanchang Univ, Dept Clin Lab, Jiangxi Prov Key Lab Lab Med, Affiliated Hosp 2, 1 Min De Rd, Nanchang 330006, Jiangxi, Peoples R China

[2] Nanchang Univ, Dept Clin Lab, Affiliated Hosp 1, 17 Yong Wai Rd, Nanchang 330006, Jiangxi, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 521卷 / 03期

基金：

中国国家自然科学基金;

关键词：

K562; cells; Exosomes; miR-711; Bone marrow mesenchymal stem cells; CHRONIC MYELOID-LEUKEMIA; MICROENVIRONMENT; TRANSPLANTATION; ANGIOGENESIS; ENGRAFTMENT;

D O I：

10.1016/j.bbrc.2019.10.096

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A failure of bone marrow mesenchymal stem cells (BM-MSCs) to adhere to hematopoietic cells is an essential cause of the progression of chronic myelogenous leukemia and is also a cause of failure of bone marrow (BM) transplantation, but the exact mechanisms of this have not been fully elucidated. Recent studies have indicated that microRNAs (miRNAs) are contained in leukemia-derived exosomes and are involved in modulating the BM microenvironment. In this study, we found that K562 cell-derived exosomes transfer miR-711 to BM-MSCs and suppress the adhesive function of BM-MSCs. Using qRT-PCR, we also confirmed a significantly higher level of miR-711 in exosomes derived from K562 cells than in exosomes derived from parental cells. The BM-MSCs co-cultured with exosomes derived from K562 cells showed a lower adhesion rate than did controls. We further demonstrated that exosomal transfer of miR-711 induced decreased adhesive abilities by inhibiting expression of adhesion molecule CD44 in BM-MSCs. In conclusion, our study reveals that K562 cell-derived exosomal miR-711 can be transferred to BM-MSCs and weaken adhesive abilities by silencing the expression of the adhesion molecule CD44. (C) 2019 Published by Elsevier Inc.

引用

页码：584 / 589

页数：6

共 25 条

[1] Mesenchymal stem cell secretes microparticles enriched in pre-microRNAs [J].