In Situ Fluorogenic Reaction Generated via Ascorbic Acid for the Construction of Universal Sensing Platform

被引：27
作者：
Fan, Yongchao [1 ,2 ]
Lv, Mengmeng [1 ]
Xue, Yuan [1 ,2 ]
Li, Jing [1 ,2 ]
Wang, Erkang [1 ,2 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
[2] Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
来源：
ANALYTICAL CHEMISTRY | 2021年 / 93卷 / 17期
关键词：
ALKALINE-PHOSPHATASE ACTIVITY; CARCINOEMBRYONIC ANTIGEN CEA; SENSITIVE DETECTION; FLUORESCENCE ASSAY; PLASMONIC ELISA; QUANTUM DOTS; NANOPARTICLES; IMMUNOASSAY; POLYDOPAMINE; BIOSENSOR;
D O I：
10.1021/acs.analchem.1c00967
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A highly fluorescent emission reaction between terephthalic acid (PTA) and ascorbic acid (AA) via simple control of the reaction temperature was first revealed with the detailed formation mechanism and various characterizations including electron paramagnetic resonance and mass spectrometry. Based on the AA-responsive emission, the alkaline phosphatase (ALP) triggered the transformation of L-ascorbic acid 2-phosphate trisodium salt to AA was integrated with the present system for developing a sensitive, selective, and universal platform. The monitoring of the activity of ALP and the fabrication of ALP-based enzyme-linked immunoassay (ELISA) with carcinoembryonic antigen (CEA) as the model target was performed. The fluorescence intensity correlated well to the CEA concentration in the ranges of 0.25-30 ng/mL, with a detection limit of 0.08 ng/mL. Such a facile protocol based on the fluorescent reaction between PTA and AA without the assistance of catalysis of nanomaterials avoided the laborious synthesis procedure and provided a direct strategy for the early clinical diagnosis coupled with ALP-related catalysis.
引用
收藏
页码：6873 / 6880
页数：8
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