New enzyme belonging to the family of molybdenum-free nitrate reductases

被引：35
作者：
Antipov, AN
Sorokin, DY
L'Vov, NP
Kuenen, JG
机构：
[1] Russian Acad Sci, Inst Microbiol, Moscow 117811, Russia
[2] Russian Acad Sci, AN Bach Inst Biochem, Moscow 119071, Russia
[3] Delft Univ Technol, Kluyver Lab Biotechnol, NL-2628 BC Delft, Netherlands
来源：
BIOCHEMICAL JOURNAL | 2003年 / 369卷
关键词：
haem-containing enzyme; nitrate reductase; nitrite reductase; vanadium-containing enzyme;
D O I：
10.1042/BJ20021193
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel molybdenum-free nitrate reductase was isolated from the obligate chemolithoautotrophic and facultative anaerobic, (halo)alkaliphilic sulphur-oxidizing bacterium Thioalkalivibrio nitratireducens strain ALEN 2. The enzyme was found to contain vanadium and haem c as cofactors. Its native molecular mass was determined as 195 kDa, and the enzyme consists of four identical subunits with apparent molecular masses of 57 kDa. Apart from nitrate, the enzyme can utilize nitrite, chlorate, bromate, selenate and sulphite as electron acceptors. Moreover, it also has a haloperoxidase activity.
引用
收藏
页码：185 / 189
页数：5
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