Use of an indirect enzyme-linked immunosorbent assay for detection of antibodies in sheep naturally infected with Salmonella Abortusovis

被引：3

作者：

Wirz-Dittus, Sophie ^{[1
,3
]}

Belloy, Luc ^{[1
]}

Doherr, Marcus G. ^{[2
]}

Huessy, Daniela ^{[3
]}

Sting, Reinhard ^{[4
]}

Gabioud, Patricia ^{[1
]}

Waldvogel, Andreas S. ^{[1
]}

机构：

[1] Inst Galli Valerio, Vet Diagnost Lab Canton Vaud, Lausanne, Switzerland

[2] Univ Bern, Vetsuisse Fac, Dept Clin Res & Vet Publ Hlth, Bern, Switzerland

[3] Univ Bern, Vetsuisse Fac, Inst Vet Bacteriol, Natl Ctr Zoonoses Bacterial Anim Dis & Antimicrob, Bern, Switzerland

[4] Chem & Vet Untersuchungsamt, Fellbach, Germany

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2010年 / 22卷 / 04期

关键词：

Abortion; enzyme-linked immunosorbent assay; Salmonella Abortusovis; sheep; OVIS; ABORTION; STRAIN; EWES;

D O I：

10.1177/104063871002200406

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

An indirect enzyme-linked immunosorbent assay (ELISA) was modified and validated to detect antibodies against Salmonella Abortusovis in naturally infected sheep. The ELISA was validated with 44 positive and 45 negative control serum samples. Compared with the immunoblot, the sensitivity and specificity of the assay were 98% and 100%, respectively. To follow antibody levels over time, samples from 12 infected ewes were collected at 1, 3, and 10 months after abortion. All animals showed antibody levels above the cutoff value throughout the observation period. One and 3 months after abortion, high antibody levels could be detected in all but one animal, whereas after 10 months, 9 animals had markedly lower but still positive antibody levels. The test characteristics and evidence for the persistence of detectable antibody levels in all infected animals for up to 10 months indicates that the ELISA can be used for herd surveillance testing.

引用

页码：531 / 536

页数：6

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