A Novel Reporter Rat Strain That Conditionally Expresses the Bright Red Fluorescent Protein tdTomato

被引：22

作者：

Igarashi, Hiroyuki ^{[1
,2
,3
]}

Koizumi, Kyo ^{[4
]}

Kaneko, Ryosuke ^{[5
,6
]}

Ikeda, Keiko ^{[7
]}

Egawa, Ryo ^{[4
]}

Yanagawa, Yuchio ^{[5
]}

Muramatsu, Shin-ichi ^{[8
,9
]}

Onimaru, Hiroshi ^{[10
]}

Ishizuka, Toru ^{[4
]}

Yawo, Hiromu ^{[1
,4
]}

机构：

[1] Tohoku Univ, Grad Sch Med, Dept Physiol & Pharmacol, Sendai, Miyagi 980, Japan

[2] Japan Soc Promot Sci, Chiyoda Ku, Tokyo, Japan

[3] Tohoku Univ, Div Interdisciplinary Adv Res & Educ, Sendai, Miyagi 980, Japan

[4] Tohoku Univ, Grad Sch Life Sci, Dept Dev Biol & Neurosci, Sendai, Miyagi 980, Japan

[5] Gunma Univ, Grad Sch Med, Dept Genet & Behav Neurosci, Maebashi, Gunma 371, Japan

[6] Gunma Univ, Grad Sch Med, Bioresource Ctr, Maebashi, Gunma 371, Japan

[7] Hyogo Coll Med, Div Biol, Nishinomiya, Hyogo 6638501, Japan

[8] Jichi Med Univ, Dept Med, Div Neurol, Shimotsuke, Tochigi, Japan

[9] Univ Tokyo, Inst Med Sci, Ctr Gene & Cell Therapy, Tokyo, Japan

[10] Showa Univ, Sch Med, Dept Physiol, Shinagawa Ku, Tokyo 142, Japan

来源：

PLOS ONE | 2016年 / 11卷 / 05期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

TRANSGENIC RATS; ESTABLISHMENT; SYSTEM; MICE; PHOX2B; CELLS; LACZ; TOOL;

D O I：

10.1371/journal.pone.0155687

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Despite the strength of the Cre/loxP recombination system in animal models, its application in rats trails that in mice because of the lack of relevant reporter strains. Here, we generated a floxed STOP tdTomato rat that conditionally expresses a red fluorescent protein variant (tdTomato) in the presence of exogenous Cre recombinase. The tdTomato signal vividly visualizes neurons including their projection fibers and spines without any histological enhancement. In addition, a transgenic rat line (FLAME) that ubiquitously expresses tdTomato was successfully established by injecting intracytoplasmic Cre mRNA into fertilized ova. Our rat reporter system will facilitate connectome studies as well as the visualization of the fine structures of genetically identified cells for long periods both in vivo and ex vivo. Furthermore, FLAME is an ideal model for organ transplantation research owing to improved traceability of cells/tissues.

引用

页数：16

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