Molecularly Imprinted PHEMA-Based Cryogel for Depletion of Hemoglobin from Human Blood

被引：81

作者：

Derazshamshir, Ali ^{[1
]}

Baydemir, Goezde ^{[1
]}

Andac, Muege ^{[1
]}

Say, Ridvan ^{[2
]}

Galaev, Igor Yu ^{[3
]}

Denizli, Adil ^{[1
]}

机构：

[1] Hacettepe Univ, Dept Chem, Div Biochem, TR-06532 Ankara, Turkey

[2] Anadolu Univ, Dept Chem, Eskisehir, Turkey

[3] Lund Univ, Dept Biotechnol, SE-22100 Lund, Sweden

来源：

MACROMOLECULAR CHEMISTRY AND PHYSICS | 2010年 / 211卷 / 06期

关键词：

affinity binding; cryogels; hemoglobin depletion; hydrogels; molecular recognition; AFFINITY; RECOGNITION; PROTEINS; POLYMERS; REMOVAL; CHROMATOGRAPHY; EQUILIBRIUM; ADSORPTION; SEPARATION; CAPTURE;

D O I：

10.1002/macp.200900425

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A new generation of molecularly imprinted macroporous hydrogels, so-called cryogels, were prepared for depletion of hemoglobin from human blood. Hb-PHEMAH cryogels were prepared with gel fraction yields up to 90%, and its morphology and porosity were characterized by FTIR, SEM, swelling studies, flow dynamics and surface area measurements. Selective binding experiments were performed in the presence of competitive PHEMAH cryogel exhibited a high binding capacity and selectivity for Hb in the presence of Myb and BSA. The selectivity of Hb-PHEMAH cryogel for Hb was confirmed by HPLC. Hb depletion from blood hemolysate was also studied using SDS-PAGE. Hb-PHE-MAH cryogel can be reused many times with no apparent decrease in Hb adsorption capacity.

引用

页码：657 / 668

页数：12

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