An enhanced cerium(IV)-rhodamine 6G chemiluminescence system using guest-host interactions in a lab-on-a-chip platform for estimating the total phenolic content in food samples

被引:21
作者
Al Haddabi, Buthaina [1 ]
Al Lawati, Haider A. J. [1 ]
Suliman, FakhrEldin O. [1 ]
机构
[1] Sultan Qaboos Univ, Coll Sci, Dept Chem, Box 36, Al Khoud 123, Oman
关键词
Lab on a chip; Microfluidics; Chemiluminescence; Surfactants; Rhodamine; Plant phenolics; ANTIOXIDANT CAPACITY; AGGREGATION; BEHAVIOR;
D O I
10.1016/j.talanta.2015.12.037
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Two chemiluminescence-microfiuidic (CL-MF) systems, e.g., Ce(IV)-rhodamine B (RB) and Ce(IV)-rhodamine 6G (R6G), for the determination of the total phenolic content in teas and some sweeteners were evaluated. The results indicated that the Ce(IV)-R6G system was more sensitive in comparison to the Ce (IV)-RB CL system. Therefore, a simple (CL-MF) method based on the CL of Ce(IV)-R6G was developed, and the sensitivity, selectivity and stability of this system were evaluated. Selected phenolic compounds (PCs), such as quercetin (QRC), catechin (CAT), rutin (RUT), gallic acid (GA), caffeic acid (CA) and syringic acid (SA), produced analytically useful chemiluminescence signals with low detection limits ranging from 035 nmol L-1 for QRC to 11.31 nmol L-1 for SA. The mixing sequence and the chip design were crucial, as the sensitivity and reproducibility could be substantially affected by these two factors. In addition, the anionic surfactant (i.e., sodium dodecyl sulfate (SDS)) can significantly enhance the CL signal intensity by as much as 300% for the QRC solution. Spectroscopic studies indicated that the enhancement was due to a strong guest-host interaction between the cationic R6G molecules and the anionic amphiphilic environment. Other parameters that could affect the CL intensities of the PCs were carefully optimized. Finally, the method was successfully applied to tea and sweetener samples. Six different tea samples exhibited total phenolic/antioxidant levels from 7.32 to 13.5 g per 100 g of sample with respect to GA. Four different sweetener samples were also analyzed and exhibited total phenolic/antioxidant levels from 500.9 to 3422.9 mg kg(-1) with respect to GA. The method was selective, rapid and sensitive when used to estimate the total phenolic/antioxidant level, and the results were in good agreement with those reported for honey and tea samples. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:399 / 406
页数:8
相关论文
共 24 条
[1]  
Al Lawati HAJ, 2014, ANAL METHODS-UK, V6, P7243, DOI [10.1039/C4AY00550C, 10.1039/c4ay00550c]
[2]   Flow-based analysis using microfluidics-chemiluminescence systems [J].
Al Lawati, Haider A. J. .
LUMINESCENCE, 2013, 28 (05) :618-627
[3]   High throughput method for the analysis of cetrizine hydrochloride in pharmaceutical formulations and in biological fluids using a tris(2,2′-bipyridyl)ruthenium(II)-peroxydisulphate chemiluminescence system in a two-chip device [J].
Al Lawati, Haider A. J. ;
Al Gharibi, Eiman ;
Al Kindy, Salma M. Z. ;
Suliman, Fakhr Eldin O. ;
Al-Lawati, Ali M. .
TALANTA, 2011, 85 (02) :906-912
[4]   Total polyphenol content and antioxidant capacity of commercially available tea (Camellia sinensis) in Argentina [J].
Anesini, Claudia ;
Ferraro, Graciela E. ;
Filip, Rosana .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (19) :9225-9229
[5]   Fluorescence enhancement of rhodamine 6G by forming inclusion complexes with β-cyclodextrin [J].
Bakkialakshmi, S. ;
Menaka, T. .
JOURNAL OF MOLECULAR LIQUIDS, 2011, 158 (02) :117-123
[6]   Dye-surfactant interaction in aqueous solutions [J].
Bielska, Malgorzata ;
Sobczynska, Anna ;
Prochaska, Krystyna .
DYES AND PIGMENTS, 2009, 80 (02) :201-205
[7]   Monitoring the total phenolic/antioxidant levels in wine using flow injection analysis with acidic potassium permanganate chemiluminescence detection [J].
Costin, JW ;
Barnett, NW ;
Lewis, SW ;
McGillivery, DJ .
ANALYTICA CHIMICA ACTA, 2003, 499 (1-2) :47-56
[8]   Chemiluminescence of cerium(IV)-rhodamine 6G-phenolic compound system [J].
Cui, Hua ;
Zhang, Qunlin ;
Myint, Aung ;
Ge, Xuewu ;
Liu, Lijuan .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 2006, 181 (2-3) :238-245
[9]   Thorough Study of Reactivity of Various Compound Classes toward the Folin-Ciocalteu Reagent [J].
Everette, Jace D. ;
Bryant, Quinton M. ;
Green, Ashlee M. ;
Abbey, Yvonne A. ;
Wangila, Grant W. ;
Walker, Richard B. .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (14) :8139-8144
[10]   Development and validation of a sequential-injection method with chemiluminescence detection for the high throughput assay of the total antioxidant capacity of wines [J].
Fassoula, Evdoxia ;
Economou, Anastasios ;
Calokerinos, Antony .
TALANTA, 2011, 85 (03) :1412-1418