Assessing SPP1/Osteopontin (OPN) Splice Variants and Their Association to Nonmelanoma Skin Cancer by Absolute Quantification: Identification of OPN-5 Subvariants and Their Protein Coding Potential

被引:13
作者
Chou, Chu-Fang [1 ]
Huang, Conway C. [2 ]
Bin Dabil, Noura [1 ]
Chang, Pi-Ling [1 ,2 ,3 ]
机构
[1] Univ Alabama Birmingham, Dept Nutr Sci, 1720 2nd Ave South, Birmingham, AL 35294 USA
[2] Univ Alabama Birmingham, Dept Dermatol, 1720 2nd Ave South, Birmingham, AL 35294 USA
[3] Univ Alabama Birmingham, ONeal Comprehens Canc Ctr, Birmingham, AL 35294 USA
关键词
Osteopontin; alternatively spliced variants; cutaneous squamous cell carcinoma; basal cell carcinoma; absolute quantification; OPN-5; subvariants; SQUAMOUS-CELL CARCINOMA; OSTEOPONTIN EXPRESSION; ACTINIC KERATOSIS; LOCALIZATION; SUBSTRATE; ROLES;
D O I
10.1080/07357907.2021.1933015
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The study evaluated whether SPP1/osteopontin (OPN) splice variants are differentially expressed in nonmelanoma skin cancer compared to normal skin. The absolute number of mRNA molecules of OPN-a predominated in normal skin and nonmelanoma skin cancer compared to OPN-b, OPN-c, and OPN-5. However, mRNAs of OPN-a, OPN-b, and OPN-c were expressed in higher levels in cutaneous squamous cell carcinomas (cSCCs) and basal cell carcinomas relative to normal skin. Additionally, OPN-5 expression was higher than OPN-b and OPN-c, and OPN-c, in normal skin and nonmelanoma skin cancer, respectively. Furthermore, we identified four OPN-5 splice variants, which were cloned and analyzed for protein expression.
引用
收藏
页码:559 / 570
页数:12
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