Generation of Merkel Cell Polyomavirus (MCV)-Like Particles and Their Application to Detection of MCV Antibodies

被引：98

作者：

Touze, Antoine

Gaitan, Julien

Arnold, Francoise

Cazal, Raphael

Fleury, Maxime J.

Combelas, Nicolas

Sizaret, Pierre-Yves ^{[2
,3
]}

Guyetant, Serge ^{[4
]}

Maruani, Annabel ^{[5
]}

Baay, Marc ^{[6
]}

Tognon, Mauro ^{[7
]}

Coursaget, Pierre ^{[1
]}

机构：

[1] Univ Tours, Fac Pharm Ph Maupas, INSERM, U618, F-37200 Tours, France

[2] Univ Tours, Serv Microscopie Elect, INSERM, U966, F-37200 Tours, France

[3] Univ Tours, Serv Microscopie Elect, IFR136, F-37200 Tours, France

[4] CHRU Tours, Hop Trousseau, Serv Anat & Cytol Pathol, Tours, France

[5] Univ Tours, Serv Dermatol, CHRU Tours, Hop Trousseau, F-37200 Tours, France

[6] Univ Antwerp, Lab Canc Res & Clin Oncol, Antwerp, Belgium

[7] Univ Ferrara, Sch Med, I-44100 Ferrara, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 05期

关键词：

MAJOR CAPSID PROTEIN; VIRUS-LIKE PARTICLES; CARCINOMA; BK; IDENTIFICATION; INFECTION; VARIANTS; SV40; VP1; L1;

D O I：

10.1128/JCM.01691-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genome of a new human polyomavirus, known as Merkel cell polyomavirus (MCV), has recently been reported to be integrated within the cellular DNA of Merkel cell carcinoma (MCC), a rare human skin cancer. To investigate MCV seroprevalence in the general population, we expressed three different MCV VP1 in insect cells using recombinant baculoviruses. Viruslike particles (VLPs) were obtained with only one of the three VP1 genes. High-titer antibodies against VP1 VLPs were detected in mice immunized with MCV VLPs, and limited cross-reactivity was observed with BK polyomavirus (BKV) and lymphotropic polyomavirus (LPV). MCV antibodies were detected in 77% of the general population, with no variations according to age.

引用

页码：1767 / 1770

页数：4

共 24 条

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