Nucleotide sequence variation of the major histocompatibility complex class II DQA1 gene in different cattle breeds from Nigeria and New Zealand

被引：1

作者：

Oladosu, Oyekunle J. ^{[1
]}

Haruna, Ishaku L. ^{[1
]}

Hadebe, Sibusiso A. ^{[1
]}

Zhou, Huitong ^{[1
]}

Hickford, Jon G. H. ^{[1
]}

机构：

[1] Lincoln Univ, Fac Agr & Life Sci, Lincoln 7647, New Zealand

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2021年 / 237卷

关键词：

Major histocompatibility complex (MHC); DQA1; PCR-SSCP; Polymorphism; DIVERSITY; SELECTION; LOCI; POLYMORPHISM; EVOLUTION; NUMBERS; SYSTEM; DNA;

D O I：

10.1016/j.vetimm.2021.110273

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The major histocompatibility complex (MHC) plays a role in immune response. Among other activities, the bovine MHC genes (BoLA) trigger immune responses, including the activation of antibody-producing B-cells. In this study, White Fulani (n = 24), Red Bororo (n = 5) and Holstein-White x Fulani-cross (n = 11) cattle from Nigeria, and New Zealand Holstein-Friesian x Jersey-cross (n = 40) cattle were used to investigate variability in exon 2 of BoLA-DQA1. Ten alleles were identified using a PCR-Single Strand Conformation Polymorphism (SSCP) approach and their nucleotide sequences confirmed by DNA sequencing. A total of 12.60 % of all nucleotide positions analysed were revealed to be variable and two novel BoLA-DQA1 alleles are reported here for the first time.

引用

页数：6

共 29 条

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