New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea

被引：25

作者：

Roh, Kyoung Ho ^{[2
]}

Kim, Sinyoung ^{[3
]}

Kim, Chang-Ki ^{[4
]}

Yum, Jong Hwa ^{[5
]}

Kim, Myung Sook

Yong, Dongeun ^{[1
,3
]}

Jeong, Seok Hoon ^{[3
]}

Lee, Kyungwon ^{[3
]}

Kim, June Myung ^{[6
]}

Chong, Yunsop ^{[3
]}

机构：

[1] Yonsei Univ, Coll Med, Dept Lab Med, Res Inst Bacterial Resistance, Seoul 120752, South Korea

[2] Korea Univ, Coll Med, Dept Lab Med, Seoul 136705, South Korea

[3] Yonsei Univ, Coll Med, Brain Korea Med Sci 21, Seoul 120752, South Korea

[4] Korean Inst TB, Seoul 137140, South Korea

[5] Dong Eui Univ, Dept Clin Lab Sci, Pusan 614714, South Korea

[6] Yonsei Univ, Coll Med, Dept Internal Med, Seoul 120752, South Korea

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2010年 / 66卷 / 04期

关键词：

Bacteroides fragilis; cfiA(11); Novel IS elements; Metallo-beta-lactamase; METALLO-BETA-LACTAMASE; GENE CFIA; IMIPENEM RESISTANCE; STRAINS; EXPRESSION; CEPA; IDENTIFICATION; PURIFICATION; PREVALENCE; UPSTREAM;

D O I：

10.1016/j.diagmicrobio.2009.11.003

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-beta-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA(11), and 2 novel IS elements. The cfiA(11) gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：343 / 348

页数：6

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