Determination of antibody induction by highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) vaccine: A comparison of two ELISA kits

被引：4
作者：
Ge, Meng [1 ,2 ]
Li, Run-Cheng [2 ]
Gong, Wenjie [1 ]
Tu, Changchun [1 ]
机构：
[1] Acad Mil Med Sci, Inst Mil Vet Med, Changchun 130122, Jilin, Peoples R China
[2] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2019年 / 81卷 / 08期
关键词：
antibody; ELISA; evaluation; highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) vaccine; ASSAY;
D O I：
10.1292/jvms.18-0482
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Two commercial porcine reproductive and respiratory syndrome virus (PRRSV) antibody ELISA kits (IDEXX and LSI) are currently in extensive use. To determine which kit is more suitable for the evaluation of HP-PRRSV vaccine efficacy, the two kits were used to test 546 serum samples. The agreement between the results was unsatisfactory, with a kappa statistic of 0.681 and a linear correlation coefficient of 0.665. In tests of samples from experimentally vaccinated and PRRSV-negative herds, IDEXX-ELISA identified antibody-positive conversion earlier and showed a higher specificity compared to LSI-ELISA. The serological profile obtained by neutralization testing was closer to that obtained by IDEXX-ELISA than by LSI-ELISA in the late immunization period. The findings reveal that IDEXX-ELISA is the more suitable for the evaluation of antibody response to HP-PRRSV vaccine and for guiding vaccination strategies.
引用
收藏
页码：1173 / 1176
页数：4
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