Optimization of the Expression of the Subtilisin-like Protease from Bacillus pumilus

被引：1
|
作者：
Cheremin, A. M. [1 ]
Nyamsuren, Ch [1 ]
Toimentseva, A. A. [1 ]
Sharipova, M. R. [1 ]
机构：
[1] Kazan Volga Reg Fed Univ, Kazan 420008, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2014年 / 40卷 / 06期
关键词：
subtilisin-like protease; Bacillus pumilus; optimization of expression; RESPONSE REGULATOR; RECOMBINANT STRAIN; DEGU; PROTEINASE; OPERON;
D O I：
10.1134/S1068162014060053
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The features of the expression of the subtilisin-like protease from Bacillus pumilus have been studied. Potential binding sites for the transcription factor DegU-P have been identified in the regulatory region of the gene of the enzyme, which were optimized for the interaction with this regulatory protein. The expression of the modified gene of the extracellular subtilisin-like protease has been investigated. It has been shown that the modification of one of the sites increased the expression of the protease twofold. It has been concluded that the optimization of the promoter leads to an increase in the expression of the subtilisin-like protease.
引用
收藏
页码：697 / 702
页数：6
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