Optimization of the Expression of the Subtilisin-like Protease from Bacillus pumilus

被引：1
|
作者：
Cheremin, A. M. [1 ]
Nyamsuren, Ch [1 ]
Toimentseva, A. A. [1 ]
Sharipova, M. R. [1 ]
机构：
[1] Kazan Volga Reg Fed Univ, Kazan 420008, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2014年 / 40卷 / 06期
关键词：
subtilisin-like protease; Bacillus pumilus; optimization of expression; RESPONSE REGULATOR; RECOMBINANT STRAIN; DEGU; PROTEINASE; OPERON;
D O I：
10.1134/S1068162014060053
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The features of the expression of the subtilisin-like protease from Bacillus pumilus have been studied. Potential binding sites for the transcription factor DegU-P have been identified in the regulatory region of the gene of the enzyme, which were optimized for the interaction with this regulatory protein. The expression of the modified gene of the extracellular subtilisin-like protease has been investigated. It has been shown that the modification of one of the sites increased the expression of the protease twofold. It has been concluded that the optimization of the promoter leads to an increase in the expression of the subtilisin-like protease.
引用
收藏
页码：697 / 702
页数：6
相关论文
共 50 条
  • [1] Optimization of the expression of the subtilisin-like protease from Bacillus pumilus
    A. M. Cheremin
    Ch. Nyamsuren
    A. A. Toimentseva
    M. R. Sharipova
    Russian Journal of Bioorganic Chemistry, 2014, 40 : 697 - 702
  • [2] Purification and molecular characterization of subtilisin-like alkaline protease BPP-A from Bacillus pumilus strain MS-1
    Miyaji, T
    Otta, Y
    Nakagawa, T
    Watanabe, T
    Niimura, Y
    Tomizuka, N
    LETTERS IN APPLIED MICROBIOLOGY, 2006, 42 (03) : 242 - 247
  • [3] Conditions of the biosynthesis of an extracellular subtilisin-like proteinase by Bacillus pumilus KMM 62
    Malikova, L. A.
    Mardanova, A. M.
    Sokolova, O. V.
    Balaban, N. P.
    Rudenskaya, G. N.
    Sharipova, M. R.
    MICROBIOLOGY, 2007, 76 (03) : 273 - 279
  • [4] Bacillus Intermedius with a Knocked-Out Gene of Subtilisin-Like Protease
    Toymentseva, A. A.
    Karimova, M. R.
    Rizvanov, A. A.
    Shagimardanova, E. I.
    Sharipova, M. R.
    UCHENYE ZAPISKI KAZANSKOGO UNIVERSITETA-SERIYA ESTESTVENNYE NAUKI, 2010, 152 (04): : 143 - 155
  • [5] Subtilisin-like proteinase secreted by the Bacillus pumilus KMM 62 strain at different growth stages
    A. M. Mardanova
    L. A. Malikova
    N. P. Balaban
    N. M. Zamatlyutdinova
    M. R. Sharipova
    Russian Journal of Bioorganic Chemistry, 2012, 38 : 203 - 210
  • [6] Subtilisin-Like Proteinase Secreted by the Bacillus pumilus KMM 62 Strain at Different Growth Stages
    Mardanova, A. M.
    Malikova, L. A.
    Balaban, N. P.
    Zamatlyutdinova, N. M.
    Sharipova, M. R.
    RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, 2012, 38 (02) : 203 - 210
  • [7] Expression and characterization of catalytic domain of Plasmodium falciparum subtilisin-like protease 3
    Alam, Asrar
    Bhatnagar, Raj K.
    Chauhan, Virander S.
    MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2012, 183 (01) : 84 - 89
  • [8] Identification of a subtilisin-like protease in seeds of developing tung fruits
    Dyer, JM
    Chapital, DC
    Lax, AR
    Pepperman, AB
    JOURNAL OF PLANT PHYSIOLOGY, 1999, 155 (06) : 802 - 805
  • [9] Kinetic analysis of salting activation of a subtilisin-like halophilic protease
    Okamoto, Debora N.
    Kondo, Marcia Y.
    Santos, Jorge A. N.
    Nakajima, Sawa
    Hiraga, Kazumi
    Oda, Kohei
    Juliano, Maria A.
    Juliano, Luiz
    Gouvea, Iuri E.
    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2009, 1794 (02): : 367 - 373
  • [10] Purification and characterization of a fibrinolytic subtilisin-like protease of Bacillus subtilis TP-6 from an Indonesian fermented soybean, Tempeh
    Kim, Seong-Bo
    Lee, Dong-Woo
    Cheigh, Chan-Ick
    Choe, Eun-Ah
    Lee, Sang-Jae
    Hong, Young-Ho
    Choi, Hak-Jong
    Pyun, Yu-Ryang
    JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2006, 33 (06) : 436 - 444
12345